Eblé Phaedra L, Bouma A, de Bruin M G M, van Hemert-Kluitenberg F, van Oirschot J T, Dekker A
Central Institute of Animal Disease Control , P.O. Box 2004, 8203 AA Lelystad, The Netherlands.
Vaccine. 2004 Mar 29;22(11-12):1372-8. doi: 10.1016/j.vaccine.2003.11.003.
The objective of this study was to investigate whether and at what time interval could vaccination reduce transmission of foot-and-mouth disease virus (FMDV) among pigs. Reduction of virus transmission by vaccination was determined experimentally. Transmission of FMDV was studied in three groups of ten pigs: one non-vaccinated group and two groups that were vaccinated 7 days (-7 dpi) and 14 days before inoculation (-14 dpi), respectively. Five randomly selected pigs from each group were inoculated with FMDV type O Taiwan, while the other five pigs left in the groups were exposed to the inoculated pigs by direct contact. Clinical signs were recorded, virus isolation and RT-PCR were carried out on oropharyngeal fluid (OPF), and the neutralizing antibody titres and the antibody response against non-structural (NS) proteins of FMDV were determined. No virus transmission was observed in the -14 dpi group, whereas virus transmission was observed in all contact pigs affecting both the non-vaccinated and the -7 dpi group. The reproduction ratio R in the -14 dpi vaccinated group was significantly lower than that of the non-vaccinated group. This study confirms the potential of vaccination as an important tool to reduce transmission of FMDV.
本研究的目的是调查接种疫苗是否以及在何种时间间隔可以减少口蹄疫病毒（FMDV）在猪群中的传播。通过实验确定接种疫苗对病毒传播的减少作用。在三组每组十头猪中研究FMDV的传播：一组未接种疫苗，另外两组分别在接种前7天（-7 dpi）和14天（-14 dpi）接种疫苗。每组随机选择五头猪接种O型台湾口蹄疫病毒，而留在组内的另外五头猪通过直接接触暴露于接种的猪。记录临床症状，对口咽液（OPF）进行病毒分离和RT-PCR，并测定中和抗体效价以及针对FMDV非结构（NS）蛋白的抗体反应。在-14 dpi组中未观察到病毒传播，而在所有接触猪中均观察到病毒传播，影响了未接种疫苗组和-7 dpi组。-14 dpi接种疫苗组的繁殖率R显著低于未接种疫苗组。本研究证实了接种疫苗作为减少FMDV传播的重要工具的潜力。
