不丹人群样本中的26个位点Y染色体短串联重复序列分型
26-Locus Y-STR typing in a Bhutanese population sample.
作者信息
Parkin Emma J, Kraayenbrink Thirsa, van Driem George L, Tshering Of Gaselô Karma, de Knijff Peter, Jobling Mark A
机构信息
Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, UK.
出版信息
Forensic Sci Int. 2006 Aug 10;161(1):1-7. doi: 10.1016/j.forsciint.2005.10.008. Epub 2005 Nov 14.
26 Y chromosome short tandem repeat (STR) loci were amplified in a sample of 856 unrelated males from Bhutan, using two multiplex polymerase chain reaction (PCR) assays. The first multiplex is the Y-STR 20plex described by Butler et al. [J.M. Butler, R. Schoske, P.M. Vallone, M.C. Kline, A.J. Redd, M.F. Hammer, A novel multiplex for simultaneous amplification of 20 Y chromosome STR markers, Forensic Sci. Int. 129 (2002) 10-24], and the second is a novel (but overlapping) 14plex that targets six additional Y-STRs (DYS425, DYS434, DYS435, DYS436, DYS461, DYS462) and also amplifies the amelogenin locus. The 26-loci give a discriminating power of 0.9957, though even at this resolution one haplotype occurs 24 times. We identify novel alleles at five loci and microvariants at a further three, which were characterised by sequencing. Extended (11-locus) haplotypes for these samples have been submitted to the Y-STR Haplotype Reference Database (YHRD).
使用两种多重聚合酶链反应（PCR）检测方法，对来自不丹的856名无亲缘关系男性样本中的26个Y染色体短串联重复序列（STR）位点进行了扩增。第一种多重检测方法是巴特勒等人描述的Y-STR 20重检测法[J.M.巴特勒、R.肖斯克、P.M.瓦隆、M.C.克莱恩、A.J.雷德、M.F.哈默，一种用于同时扩增20个Y染色体STR标记的新型多重检测法，《法医学国际》129（2002）10 - 24]，第二种是一种新型（但有重叠）的14重检测法，它针对另外六个Y-STR（DYS425、DYS434、DYS435、DYS436、DYS461、DYS462），并且还扩增牙釉蛋白基因座。这26个位点的鉴别力为0.9957，不过即使在这种分辨率下，一种单倍型出现了24次。我们在五个位点鉴定出了新等位基因，在另外三个位点鉴定出了微变体，并通过测序对其进行了特征描述。这些样本的扩展（11个位点）单倍型已提交至Y-STR单倍型参考数据库（YHRD）。
Zotero 插件