Page K M, Mallett C D, Page M R
Dagenham Research Centre, Rhône Poulenc Rorer Ltd., Essex, UK.
J Chromatogr. 1992 Mar 27;575(2):319-24. doi: 10.1016/0378-4347(92)80165-m.
A method is described for the determination of the novel hydroxymethyl glutaryl coenzyme A reductase inhibitor RP 61969 (I) and its hydrolysis product, the dihydroxy acid RP 62420 (II), in human plasma. A structural isomer of I is used as internal standard. Both I and II were extracted from acidified plasma with diethyl ether. The dried residues were reconstituted in the high-performance liquid chromatography mobile phase and chromatographed on a 5 microns ODS2 column. The mobile phase used was aqueous dipotassium phosphate +tetra-n-butyl ammonium bromide (both 10 mM)-acetonitrile-methanol (60:40:5, v/v). At a flow-rate of 1.5 ml min-1 and ambient temperature, the retention time of II is 3.5 min, that of the internal standard is 5 min, and that of I is 8 min. The method has been validated and applied to the assay of plasma samples resulting from a cell-plasma distribution experiment in human whole blood.
描述了一种测定人血浆中新型羟甲基戊二酰辅酶A还原酶抑制剂RP 61969（I）及其水解产物二羟基酸RP 62420（II）的方法。I的一种结构异构体用作内标。I和II均用乙醚从酸化血浆中萃取。干燥后的残渣用高效液相色谱流动相复溶，并在5微米的ODS2柱上进行色谱分析。所用流动相为磷酸氢二钾水溶液+四丁基溴化铵（均为10 mM）-乙腈-甲醇（60:40:5，v/v）。在流速为1.5 ml·min-1和室温下，II的保留时间为3.5分钟，内标的保留时间为5分钟，I的保留时间为8分钟。该方法已经过验证，并应用于人体全血细胞-血浆分布实验所得血浆样品的测定。
