Lindberg R L
J Chromatogr. 1985 Jun 14;341(2):333-9. doi: 10.1016/s0378-4347(00)84046-3.
A selective high-performance liquid chromatographic method for the determination of the three metabolites of nomifensine in human plasma is described. All metabolites and the internal standard, mexiletine, are extracted with diethyl ether and then back-extracted into an acidic aqueous phase. After subsequent extraction into diethyl ether the metabolites are analysed by high-performance liquid chromatography. A reversed-phase C18 column is used with a mobile phase of dioxane-methanol-potassium phosphate buffer (pH 2.25). The sensitivity of the method is 0.007 micromol/l for all metabolites. Extraction efficiencies are 84.6%, 75.8%, and 78.2% for 4'-hydroxynomifensine, 4'-hydroxy-3'-methoxynomifensine and 3'-hydroxy-4'-methoxynomifensine, respectively. The reproducibility of the method is good, the coefficients of variation (%) varying between 2.1% and 9.9% in the concentration range 0.05-1.00 micromol/l. The procedure was applied to human plasma samples from a volunteer who had received a single oral dose of nomifensine. The method is accurate and sensitive for pharmacokinetic studies on the metabolites of nomifensine.
本文描述了一种用于测定人血浆中诺米芬辛三种代谢物的选择性高效液相色谱法。所有代谢物和内标美西律均用乙醚萃取，然后反萃取到酸性水相中。随后再用乙醚萃取后，代谢物通过高效液相色谱法进行分析。使用反相C18柱，流动相为二氧六环 - 甲醇 - 磷酸钾缓冲液（pH 2.25）。该方法对所有代谢物的灵敏度为0.007微摩尔/升。4'-羟基诺米芬辛、4'-羟基-3'-甲氧基诺米芬辛和3'-羟基-4'-甲氧基诺米芬辛的萃取效率分别为84.6%、75.8%和78.2%。该方法的重现性良好，在0.05 - 1.00微摩尔/升的浓度范围内，变异系数（%）在2.1%至9.9%之间变化。该方法应用于一名口服单剂量诺米芬辛志愿者的人血浆样本。该方法对诺米芬辛代谢物的药代动力学研究准确且灵敏。
