Kaye M, Silverton S, Rosenthall L
J Nucl Med. 1975 Jan;16(1):40-5.
In rats with induced rickets, the uptake of 99mTcO4 and 99mTc-pyrophosphate per gram of bone was increased as compared with weight-matched controls. However, the uptake of radioactive calcium and 32P-pyrophosphate was similar in both rachitic and control animals, suggesting that the 99mTc label conferred specificity and favored the rachitic lesions. Employing the rat tibia in an in vitro system, 99mTcO4 uptake was predominantly in the organic bone matrix; radioactive calcium, 32P-pyrophosphate, or 14C-diphosphonate uptake was mainly in the bone mineral; and 99mTc-pyrophosphate, 99mTc-diphosphonate, and 99mTc-polyphosphate were found in both mineral and organic phases. By removal of both mineral and polysaccharide and by using agents that altered the degree of collagen fibril cross-linking, evidence was obtained suggesting the 99mTcO4 and 99mTc-pyrophosphate are preferentially bound by immature collagen.
在诱导性佝偻病大鼠中，与体重匹配的对照组相比，每克骨骼对99m锝酸盐和99m锝-焦磷酸盐的摄取增加。然而，佝偻病大鼠和对照动物对放射性钙和32P-焦磷酸盐的摄取相似，这表明99m锝标记具有特异性，且有利于佝偻病病变。在体外系统中使用大鼠胫骨，99m锝酸盐摄取主要在有机骨基质中；放射性钙、32P-焦磷酸盐或14C-二膦酸盐摄取主要在骨矿物质中；而99m锝-焦磷酸盐、99m锝-二膦酸盐和99m锝-多磷酸盐在矿物质和有机相中均有发现。通过去除矿物质和多糖，并使用改变胶原纤维交联程度的试剂，获得的证据表明99m锝酸盐和99m锝-焦磷酸盐优先与未成熟胶原结合。
