McAnulty Steven R, McAnulty Lisa S, Nieman David C, Morrow Jason D, Utter Alan C, Dumke Charles L
Department of Health, Leisure and Exercise Science, Appalachian State University, Boone, NC 28608, USA.
Free Radic Res. 2005 Nov;39(11):1219-24. doi: 10.1080/10725760500317536.
Some research studies have produced data indicating that resistance exercise induces oxidative stress, despite minimal increases in VO(2). These studies have primarily relied on oxidative stress markers with low sensitivity and debatable reliability. However, F(2)-isoprostanes as measured by gas chromatography mass spectrometry are considered to be a reliable and precise indicator of oxidative stress. Carbohydrate ingestion during exercise is associated with reduced levels of stress hormones, which may influence oxidative stress and plasma antioxidant potential. Therefore, the purpose of this study was to investigate the influence of carbohydrate ingestion during resistance training on F(2)-isoprostanes and plasma antioxidant potential. Thirty strength-trained subjects were randomized to carbohydrate (CHO) or placebo (PLA) groups that lifted weights for 2 h. Subjects received 10 ml kg(- 1) h(- 1) CHO (6%) or PLA beverages during the exercise. Blood and vastus lateralis muscle biopsy samples were collected before and after exercise and analyzed for cortisol as a marker of general stress, F(2)-isoprostanes as a measure of oxidative stress, and ferric reducing ability of plasma (FRAP) as a measure of antioxidant potential, and for muscle glycogen, respectively. Decreases in muscle glycogen content did not differ between CHO and PLA. Cortisol and FRAP increased significantly in CHO and PLA (P = 0.008 and 0.044, respectively), but the pattern of change was not different between groups. F(2)-isoprostanes were unaffected by exercise. These results indicate that exhaustive resistance exercise and carbohydrate ingestion have no effect on oxidative stress or plasma antioxidant potential in trained subjects.
一些研究得出的数据表明，尽管耗氧量增加极少，但抗阻运动仍会引发氧化应激。这些研究主要依赖于灵敏度较低且可靠性存疑的氧化应激标志物。然而，通过气相色谱质谱法测定的F(2)-异前列腺素被认为是氧化应激的可靠且精确的指标。运动期间摄入碳水化合物与应激激素水平降低有关，这可能会影响氧化应激和血浆抗氧化能力。因此，本研究的目的是探讨抗阻训练期间摄入碳水化合物对F(2)-异前列腺素和血浆抗氧化能力的影响。30名经过力量训练的受试者被随机分为碳水化合物（CHO）组或安慰剂（PLA）组，进行2小时的举重训练。受试者在运动期间接受10 ml·kg(-1)·h(-1)的CHO（6%）或PLA饮料。在运动前后采集血液和股外侧肌活检样本，分别分析作为一般应激标志物的皮质醇、作为氧化应激指标的F(2)-异前列腺素、作为抗氧化能力指标的血浆铁还原能力（FRAP）以及肌肉糖原。CHO组和PLA组肌肉糖原含量的下降没有差异。CHO组和PLA组的皮质醇和FRAP均显著增加（分别为P = 0.008和0.044），但两组之间的变化模式没有差异。F(2)-异前列腺素不受运动影响。这些结果表明，力竭性抗阻运动和碳水化合物摄入对受过训练的受试者的氧化应激或血浆抗氧化能力没有影响。
