Gomes Juliana A S, Bahia-Oliveira Lilian M G, Rocha Manoel Otávio C, Busek Solange C U, Teixeira Mauro M, Silva João Santana, Correa-Oliveira Rodrigo
Laboratório de Imunologia Celular e Molecular-Centro de Pesquisas René Rachou/FIOCRUZ, Av. Augusto de Lima 1715, 30190-002, Belo Horizonte, MG, Brazil.
Infect Immun. 2005 Dec;73(12):7960-6. doi: 10.1128/IAI.73.12.7960-7966.2005.
Chemokines and chemokine receptors (CKRs) control the migration of leukocytes during the inflammatory process and are important immunological markers of type 1 (CCR5 and CXCR3) and type 2 (CCR3 and CCR4) responses. The coexpression of CKRs (CCR2, CCR3, CCR5, CXCR3, and CXCR4) and intracellular cytokines (interleukin-10 [IL-10], IL-4, tumor necrosis factor alpha [TNF-alpha], and gamma interferon [IFN-gamma]) on T CD4+ and CD8+ peripheral cells from individuals with indeterminate (IND) or cardiac (CARD) clinical forms of Chagas' disease after in vitro stimulation with Trypanosoma cruzi antigens, were evaluated in this study. The percentage of T CD4+ and CD8+ cells coexpressing CCR5 and IFN-gamma, CXCR3 and IFN-gamma, and CXCR3 and TNF-alpha were higher in CARD than in IND individuals; on the other hand, the percentage of T CD4+ or CD8+ cells coexpressing CCR3 and IL-10 or coexpressing CCR3 and IL-4 were lower in CARD individuals than in IND individuals. In addition, a significant positive correlation between the expression of CCR5 or CXCR3 and IFN-gamma was observed in CARD individuals contrasting with a significant positive correlation between the expression of CCR3 and IL-4 and of CCR3 and IL-10 in IND patients. These results reinforce the hypothesis that a T. cruzi-exacerbated specific type 1 immune response developed by CARD chagasic patients is associated with the development of heart pathology.
趋化因子和趋化因子受体（CKRs）在炎症过程中控制白细胞的迁移，是1型（CCR5和CXCR3）和2型（CCR3和CCR4）免疫反应的重要免疫标志物。本研究评估了克氏锥虫抗原体外刺激后，患有不确定（IND）或心脏（CARD）临床型恰加斯病个体的外周T CD4+和CD8+细胞上CKRs（CCR2、CCR3、CCR5、CXCR3和CXCR4）与细胞内细胞因子（白细胞介素-10 [IL-10]、IL-4、肿瘤坏死因子α [TNF-α]和γ干扰素[IFN-γ]）的共表达情况。CARD个体中，共表达CCR5和IFN-γ、CXCR3和IFN-γ以及CXCR3和TNF-α的T CD4+和CD8+细胞百分比高于IND个体；另一方面，CARD个体中共表达CCR3和IL-10或共表达CCR3和IL-4的T CD4+或CD8+细胞百分比低于IND个体。此外，在CARD个体中观察到CCR5或CXCR3表达与IFN-γ之间存在显著正相关，而在IND患者中CCR3表达与IL-4以及CCR3表达与IL-10之间存在显著正相关。这些结果强化了这样一种假设，即CARD型恰加斯病患者由克氏锥虫加剧的特异性1型免疫反应与心脏病理的发展有关。
