Gelperin Daniel M, White Michael A, Wilkinson Martha L, Kon Yoshiko, Kung Li A, Wise Kevin J, Lopez-Hoyo Nelson, Jiang Lixia, Piccirillo Stacy, Yu Haiyuan, Gerstein Mark, Dumont Mark E, Phizicky Eric M, Snyder Michael, Grayhack Elizabeth J
Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA.
Genes Dev. 2005 Dec 1;19(23):2816-26. doi: 10.1101/gad.1362105.
Functional analysis of the proteome is an essential part of genomic research. To facilitate different proteomic approaches, a MORF (moveable ORF) library of 5854 yeast expression plasmids was constructed, each expressing a sequence-verified ORF as a C-terminal ORF fusion protein, under regulated control. Analysis of 5573 MORFs demonstrates that nearly all verified ORFs are expressed, suggests the authenticity of 48 ORFs characterized as dubious, and implicates specific processes including cytoskeletal organization and transcriptional control in growth inhibition caused by overexpression. Global analysis of glycosylated proteins identifies 109 new confirmed N-linked and 345 candidate glycoproteins, nearly doubling the known yeast glycome.
蛋白质组的功能分析是基因组研究的重要组成部分。为了促进不同的蛋白质组学方法,构建了一个包含5854个酵母表达质粒的MORF(可移动开放阅读框)文库,每个文库在调控控制下表达一个经序列验证的开放阅读框作为C末端开放阅读框融合蛋白。对5573个MORF的分析表明,几乎所有经验证的开放阅读框都能表达,表明48个被归类为可疑的开放阅读框是真实的,并暗示了包括细胞骨架组织和转录控制在内的特定过程与过表达引起的生长抑制有关。对糖基化蛋白的全局分析鉴定出109个新的已确认的N-连接糖蛋白和345个候选糖蛋白,使已知的酵母糖组几乎增加了一倍。