Beck Iris-Constanze, Bruhn Regina, Gandrass Juergen
GKSS Research Centre, Institute for Coastal Research, Max-Planck-Strasse 1, D-21502 Geesthacht, Germany.
Chemosphere. 2006 Jun;63(11):1870-8. doi: 10.1016/j.chemosphere.2005.10.022. Epub 2005 Dec 5.
In the present study, the yeast estrogen screen (YES) has been used to assess the estrogenic activity in surface waters of a coastal region in the German Baltic Sea. Solid-phase extraction using the copolymer Oasis HLB followed by a clean-up on silica was carried out on approximately 50-l water samples. From the final 400 microl extract volume, 100 microl aliquots were used for the measurement of estrogenic activity and for chemical analysis, which was performed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). From 29 samples taken during two campaigns (2003 and 2004) at five different stations 27 samples showed an estrogenic response higher than 10%. The response in the YES was expressed as measured estradiol equivalents (EEQs), which were in the range of 0.01 (Darss Peninsula) to 0.82 ng/l (Inner Wismar Bay). Samples from stations located in inner coastal waters showed higher estrogenic activities than those from outer located stations. A comparison of measured estrogenicity (YES) and calculated estrogenicity (chemical analysis) showed significant differences, probably due to the presence of anti-estrogenic compounds and/or the estrogenic activity of unknown, not identified contaminants. The main contributors to the overall estrogenic activity were synthetic and natural hormones.
在本研究中，酵母雌激素筛选法（YES）已被用于评估德国波罗的海沿海地区地表水中的雌激素活性。对约50升水样进行了使用共聚物Oasis HLB的固相萃取，随后在硅胶上进行净化处理。从最终400微升的萃取液体积中，取100微升等分试样用于雌激素活性的测定和化学分析，化学分析通过液相色谱-串联质谱联用（LC-MS/MS）进行。在2003年和2004年的两次采样活动中，在五个不同站点采集的29个样品中，有27个样品显示出高于10%的雌激素反应。YES中的反应以测得的雌二醇当量（EEQs）表示，其范围为0.01（达尔斯半岛）至0.82纳克/升（维斯马内湾）。位于内岸水域站点的样品显示出比外岸水域站点样品更高的雌激素活性。测得的雌激素性（YES）与计算得出的雌激素性（化学分析）的比较显示出显著差异，这可能是由于抗雌激素化合物的存在和/或未知的、未鉴定污染物的雌激素活性所致。总体雌激素活性的主要贡献者是合成激素和天然激素。
