原水中隐孢子虫种类和基因型的分子法医分析
Molecular forensic profiling of Cryptosporidium species and genotypes in raw water.
作者信息
Ruecker Norma J, Bounsombath Niravanh, Wallis Peter, Ong Corinne S L, Isaac-Renton Judith L, Neumann Norman F
机构信息
Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada.
出版信息
Appl Environ Microbiol. 2005 Dec;71(12):8991-4. doi: 10.1128/AEM.71.12.8991-8994.2005.
Abstract
The emerging concept of host specificity of Cryptosporidium spp. was exploited to characterize sources of fecal contamination in a watershed. A method of molecular forensic profiling of Cryptosporidium oocysts on microscope slides prepared from raw water samples processed by U.S. Environmental Protection Agency Method 1623 was developed. The method was based on a repetitive nested PCR-restriction fragment length polymorphism-DNA sequencing approach that permitted the resolution of multiple species/genotypes of Cryptosporidium in a single water sample.
摘要
隐孢子虫属宿主特异性这一新兴概念被用于确定流域粪便污染的来源。我们开发了一种分子法医分析方法，用于对经美国环境保护局方法1623处理的原水样本制备的显微镜载玻片上的隐孢子虫卵囊进行分析。该方法基于重复巢式PCR-限制性片段长度多态性- DNA测序方法，能够在单个水样中分辨出多种隐孢子虫的物种/基因型。
相似文献
1
Molecular forensic profiling of Cryptosporidium species and genotypes in raw water.原水中隐孢子虫种类和基因型的分子法医分析
Appl Environ Microbiol. 2005 Dec;71(12):8991-4. doi: 10.1128/AEM.71.12.8991-8994.2005.
2
Identification of Cryptosporidium species and genotypes in Scottish raw and drinking waters during a one-year monitoring period.在一年的监测期间，鉴定苏格兰原水和饮用水中的隐孢子虫种类和基因型。
Appl Environ Microbiol. 2010 Sep;76(17):5977-86. doi: 10.1128/AEM.00915-10. Epub 2010 Jul 16.
3
Molecular characterization of cryptosporidium oocysts in samples of raw surface water and wastewater.地表原水和废水样本中隐孢子虫卵囊的分子特征分析
Appl Environ Microbiol. 2001 Mar;67(3):1097-101. doi: 10.1128/AEM.67.3.1097-1101.2001.
4
Detection and resolution of Cryptosporidium species and species mixtures by genus-specific nested PCR-restriction fragment length polymorphism analysis, direct sequencing, and cloning.应用种特异性巢式 PCR-限制性片段长度多态性分析、直接测序和克隆技术检测和解析隐孢子虫种和种混合物。
Appl Environ Microbiol. 2011 Jun;77(12):3998-4007. doi: 10.1128/AEM.02706-10. Epub 2011 Apr 15.
5
Sources and species of cryptosporidium oocysts in the Wachusett Reservoir watershed.Wachusett水库流域隐孢子虫卵囊的来源和种类。
Appl Environ Microbiol. 2002 Feb;68(2):569-75. doi: 10.1128/AEM.68.2.569-575.2002.
6
Detection and differentiation of Cryptosporidium oocysts in water by PCR-RFLP.通过聚合酶链反应-限制性片段长度多态性技术检测和鉴别水中的隐孢子虫卵囊
Methods Mol Biol. 2004;268:163-76. doi: 10.1385/1-59259-766-1:163.
7
The detection of Cryptosporidium and the resolution of mixtures of species and genotypes from water.水中隐孢子虫的检测以及种和基因型混合物的解析。
Infect Genet Evol. 2013 Apr;15:3-9. doi: 10.1016/j.meegid.2012.09.009. Epub 2012 Oct 23.
8
Distribution of cryptosporidium genotypes in storm event water samples from three watersheds in New York.纽约三个流域暴雨事件水样中隐孢子虫基因型的分布
Appl Environ Microbiol. 2005 Aug;71(8):4446-54. doi: 10.1128/AEM.71.8.4446-4454.2005.
9
Tracking host sources of Cryptosporidium spp. in raw water for improved health risk assessment.追踪原水中隐孢子虫属的宿主来源以改进健康风险评估。
Appl Environ Microbiol. 2007 Jun;73(12):3945-57. doi: 10.1128/AEM.02788-06. Epub 2007 May 4.
10
Occurrence of Giardia and Cryptosporidium spp. in surface water supplies.贾第虫和隐孢子虫属在地表水源中的出现情况。
Appl Environ Microbiol. 1991 Sep;57(9):2610-6. doi: 10.1128/aem.57.9.2610-2616.1991.
引用本文的文献
1
CryptoGenotyper: A new bioinformatics tool for rapid identification.加密基因分型器：一种用于快速鉴定的新型生物信息学工具。
Food Waterborne Parasitol. 2021 Feb 26;23:e00115. doi: 10.1016/j.fawpar.2021.e00115. eCollection 2021 Jun.
2
Molecular detection and prevalence of Cryptosporidium spp. infections in two types of domestic farm animals in the Qinghai-Tibetan Plateau Area (QTPA) in China.中国青藏高原地区（QTPA）两种家畜中隐孢子虫属感染的分子检测及流行情况
Parasitol Res. 2018 Jan;117(1):233-239. doi: 10.1007/s00436-017-5697-2. Epub 2017 Dec 3.
3
A comprehensive method for amplicon-based and metagenomic characterization of viruses, bacteria, and eukaryotes in freshwater samples.一种用于在淡水样本中扩增子和宏基因组分析病毒、细菌和真核生物的综合方法。
Microbiome. 2016 Jul 19;4(1):20. doi: 10.1186/s40168-016-0166-1.
4
Egg intensity and freeze-thawing of fecal samples affect sensitivity of Echinococcus multilocularis detection by PCR.粪便样本的虫卵浓度和冻融处理会影响通过聚合酶链反应检测多房棘球绦虫的灵敏度。
Parasitol Res. 2014 Oct;113(10):3867-73. doi: 10.1007/s00436-014-4055-x. Epub 2014 Aug 2.
5
Assessment of Giardia and Cryptosporidium spp. as a microbial source tracking tool for surface water: application in a mixed-use watershed.贾第虫属和隐孢子虫属作为地表水微生物源追踪工具的评估：在混合用途流域中的应用
Appl Environ Microbiol. 2014 Apr;80(8):2328-36. doi: 10.1128/AEM.02037-13. Epub 2014 Jan 24.
6
Occurrence of waterborne pathogens and Escherichia coli at offshore drinking water intakes in lake Ontario.安大略湖近海饮用水进水口的水源性病原体和大肠杆菌的出现情况。
Appl Environ Microbiol. 2013 Oct;79(19):5799-813. doi: 10.1128/AEM.00870-13. Epub 2013 Jul 8.
7
Occurrence, source, and human infection potential of cryptosporidium and Giardia spp. in source and tap water in shanghai, china.中国上海水源水和自来水中隐孢子虫和贾第虫属的发生、来源及对人类的感染潜力。
Appl Environ Microbiol. 2011 Jun;77(11):3609-16. doi: 10.1128/AEM.00146-11. Epub 2011 Apr 15.
8
Detection and resolution of Cryptosporidium species and species mixtures by genus-specific nested PCR-restriction fragment length polymorphism analysis, direct sequencing, and cloning.应用种特异性巢式 PCR-限制性片段长度多态性分析、直接测序和克隆技术检测和解析隐孢子虫种和种混合物。
Appl Environ Microbiol. 2011 Jun;77(12):3998-4007. doi: 10.1128/AEM.02706-10. Epub 2011 Apr 15.
9
Identification of Cryptosporidium species and genotypes in Scottish raw and drinking waters during a one-year monitoring period.在一年的监测期间，鉴定苏格兰原水和饮用水中的隐孢子虫种类和基因型。
Appl Environ Microbiol. 2010 Sep;76(17):5977-86. doi: 10.1128/AEM.00915-10. Epub 2010 Jul 16.
10
Cryptosporidium source tracking in the Potomac River watershed.波托马克河流域隐孢子虫的溯源追踪
Appl Environ Microbiol. 2008 Nov;74(21):6495-504. doi: 10.1128/AEM.01345-08. Epub 2008 Sep 5.
本文引用的文献
1
Genotypes of Cryptosporidium species infecting fur-bearing mammals differ from those of species infecting humans.感染毛皮哺乳动物的隐孢子虫物种的基因型与感染人类的物种的基因型不同。
Appl Environ Microbiol. 2004 Dec;70(12):7574-7. doi: 10.1128/AEM.70.12.7574-7577.2004.
2
Cryptosporidium suis n. sp. (Apicomplexa: Cryptosporidiidae) in pigs (Sus scrofa).猪隐孢子虫新种（顶复门：隐孢子虫科）在猪（野猪）中的发现
J Parasitol. 2004 Aug;90(4):769-73. doi: 10.1645/GE-202R1.
3
PCR-mediated recombination between Cryptosporidium spp. of lizards and snakes.
J Eukaryot Microbiol. 2003;50 Suppl:563-5. doi: 10.1111/j.1550-7408.2003.tb00630.x.
4
Cryptosporidium taxonomy: recent advances and implications for public health.隐孢子虫分类学：最新进展及其对公共卫生的影响
Clin Microbiol Rev. 2004 Jan;17(1):72-97. doi: 10.1128/CMR.17.1.72-97.2004.
5
Host adaptation and host-parasite co-evolution in Cryptosporidium: implications for taxonomy and public health.隐孢子虫的宿主适应性及宿主-寄生虫协同进化：对分类学和公共卫生的影响
Int J Parasitol. 2002 Dec 19;32(14):1773-85. doi: 10.1016/s0020-7519(02)00197-2.
6
Detection of eight Cryptosporidium genotypes in surface and waste waters in Europe.
Parasitology. 2002 Apr;124(Pt 4):359-68. doi: 10.1017/s0031182001001317.
7
Sources and species of cryptosporidium oocysts in the Wachusett Reservoir watershed.Wachusett水库流域隐孢子虫卵囊的来源和种类。
Appl Environ Microbiol. 2002 Feb;68(2):569-75. doi: 10.1128/AEM.68.2.569-575.2002.
8
Molecular characterization of cryptosporidium oocysts in samples of raw surface water and wastewater.地表原水和废水样本中隐孢子虫卵囊的分子特征分析
Appl Environ Microbiol. 2001 Mar;67(3):1097-101. doi: 10.1128/AEM.67.3.1097-1101.2001.
9
Identification of species and sources of Cryptosporidium oocysts in storm waters with a small-subunit rRNA-based diagnostic and genotyping tool.使用基于小亚基核糖体RNA的诊断和基因分型工具鉴定暴雨水中隐孢子虫卵囊的种类和来源。
Appl Environ Microbiol. 2000 Dec;66(12):5492-8. doi: 10.1128/AEM.66.12.5492-5498.2000.
10
Evaluation of Cryptosporidium parvum genotyping techniques.微小隐孢子虫基因分型技术评估
Appl Environ Microbiol. 1999 Oct;65(10):4431-5. doi: 10.1128/AEM.65.10.4431-4435.1999.
Zotero 插件