猿猴病毒40温度敏感突变体之间的互补产物。
Products of complementation between temperature-sensitive mutants of simian virus 40.
作者信息
Chou J Y, Martin R G
出版信息
J Virol. 1975 Jan;15(1):127-36. doi: 10.1128/JVI.15.1.127-136.1975.
Abstract
Temperature-sensitive mutants of the D complementation group of simian virus 40 exhibit delayed complementation. Analysis of the thermal stability, kinetic profiles in temperature shift experiments, and progeny of complementation have led to the hypothesis that delayed complementation is not true complementation, but the result of a very low level of leakiness, followed by phenotypic mixing of the progeny D mutants. This hypothesis is consistent with the proposal that D mutants are defective in uncoating. In the course of these experiments, it was observed that fresh medium suppresses the growth of D mutants at the restrictive temperature.
摘要
猿猴病毒40的D互补组温度敏感突变体表现出延迟互补。对热稳定性、温度转换实验中的动力学曲线以及互补后代的分析得出了这样一个假说：延迟互补并非真正的互补，而是极低水平渗漏的结果，随后是后代D突变体的表型混合。这一假说与D突变体在脱壳方面存在缺陷的观点一致。在这些实验过程中，观察到新鲜培养基在限制温度下会抑制D突变体的生长。
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