由埃及嗜血杆菌的一种限制酶（核酸内切酶R-HaeIII）切割产生的多瘤病毒DNA片段的物理图谱。
Physical map of polyoma viral DNA fragments produced by cleavage with a restriction enzyme from Haemophilus aegyptius, endonuclease R-HaeIII.
作者信息
Summers J
出版信息
J Virol. 1975 Apr;15(4):946-53. doi: 10.1128/JVI.15.4.946-953.1975.
Abstract
Digestion of polyoma viral DNA with a restriction enzyme from Haemophilus aegyptius generates at least 22 unique fragments. The fragments have been characterized with respect to size and physical order on the polyoma genome, and the 5' to 3' orientation of the (+) and (-) strands has been determined. A method for specific radiolabeling of adjacent fragments was employed to establish the fragment order. This technique may be useful for ordering the fragments produced by digestion of complex DNAs.
摘要
用来自埃及嗜血杆菌的一种限制酶消化多瘤病毒DNA可产生至少22个独特的片段。这些片段已根据其大小、在多瘤病毒基因组上的物理顺序进行了表征，并且已经确定了（+）链和（-）链从5'到3'的方向。采用一种对相邻片段进行特异性放射性标记的方法来确定片段顺序。该技术可能有助于对复杂DNA消化产生的片段进行排序。
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