禽成髓细胞瘤病毒RNA中与白血病鸡细胞DNA快速杂交的序列散布情况。
Interspersion of sequences in avian myeloblastosis virus rna that rapidly hybridize with leukemic chicken cell DNA.
作者信息
Drohan W N, Shoyab M, Wall R, Baluda M A
出版信息
J Virol. 1975 Mar;15(3):550-5. doi: 10.1128/JVI.15.3.550-555.1975.
Abstract
Liquid hybridization of progressively smaller fragments (35S, 27S, 15.5S, 12.5S, and 8S) of poly(A)-selected avian myeloblastosis virus RNA with excess DNA from leukemic chicken myeloblasts revealed that all sizes of RNA contained sequences complementary to both slowly and rapidly hybridizing cellular DNA sequences. Apparently, the RNA sequences which hybridize rapidly with excesses of cellular DNA are not restricted to any one region of the avian myeloblastosis virus 35S RNA. Instead, they appear to be randomly distributed over the entire 35S avian myeloblastosis virus RNA molecule with some positioned within 200 nucleotides of the poly(A) tract at the 3' end of the RNA.
摘要
对经聚腺苷酸选择的禽成髓细胞瘤病毒RNA的逐渐变小的片段（35S、27S、15.5S、12.5S和8S）与来自白血病鸡成髓细胞的过量DNA进行液相杂交，结果显示所有大小的RNA都含有与细胞DNA序列中缓慢和快速杂交序列互补的序列。显然，与过量细胞DNA快速杂交的RNA序列并不局限于禽成髓细胞瘤病毒35S RNA的任何一个区域。相反，它们似乎随机分布在整个35S禽成髓细胞瘤病毒RNA分子上，有些位于RNA 3'端聚腺苷酸序列200个核苷酸范围内。
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本文引用的文献
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Covalent linkage between ribonucleic Acid primer and deoxyribonucleic Acid product of the avian myeloblastosis virus deoxyribonucleic Acid polymerase.禽成髓细胞瘤病毒脱氧核糖核酸聚合酶的核糖核酸引物与脱氧核糖核酸产物之间的共价连接。
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Hybridization properties of DNA sequences directing the synthesis of messenger RNA and heterogeneous nuclear RNA.指导信使核糖核酸和不均一核核糖核酸合成的DNA序列的杂交特性。
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