Hillier Ladeana W, Coulson Alan, Murray John I, Bao Zhirong, Sulston John E, Waterston Robert H
Genome Sequencing Center, Washington University School of Medicine, St. Louis, Missouri 63108, USA.
Genome Res. 2005 Dec;15(12):1651-60. doi: 10.1101/gr.3729105.
The Caenorhabditis elegans genome sequence is now complete, fully contiguous telomere to telomere and totaling 100,291,840 bp. The sequence has catalyzed the collection of systematic data sets and analyses, including a curated set of 19,735 protein-coding genes--with >90% directly supported by experimental evidence--and >1300 noncoding RNA genes. High-throughput efforts are under way to complete the gene sets, along with studies to characterize gene expression, function, and regulation on a genome-wide scale. The success of the worm project has had a profound effect on genome sequencing and on genomics more broadly. We now have a solid platform on which to build toward the lofty goal of a true molecular understanding of worm biology with all its implications including those for human health.
秀丽隐杆线虫的基因组序列现已完成，从端粒到端粒完全连续，总长100,291,840碱基对。该序列推动了系统数据集的收集和分析，包括一组经过精心整理的19,735个蛋白质编码基因（其中90%以上有实验证据直接支持）以及1300多个非编码RNA基因。目前正在进行高通量研究以完善基因集，同时也在开展全基因组范围内表征基因表达、功能和调控的研究。线虫项目的成功对基因组测序乃至更广泛的基因组学产生了深远影响。我们现在有了一个坚实的平台，可朝着真正从分子层面理解线虫生物学及其所有影响（包括对人类健康的影响）这一崇高目标迈进。
