Stamp G W, Poulsom R, Chung L P, Keshav S, Jeffery R E, Longcroft J A, Pignatelli M, Wright N A
Histopathology Unit in Sity Hybridisation Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London.
Gastroenterology. 1992 Aug;103(2):532-8. doi: 10.1016/0016-5085(92)90843-n.
Riboprobe in situ hybridization (rISH) demonstrates active lysozyme synthesis in ulcerative colitis and Crohn's disease. Maximal labeling was seen in Paneth cells, macrophages, and granulomas. Diffuse infiltration of the mucosa by lysozyme-rich polymorphs characterizes ulcerative colitis but obscures reactivity in other cell lineages in immunohistochemical studies; lysozyme mRNA is not detected in polymorphs, rISH giving a clearer picture than immunohistochemical studies of the active synthesis of lysozyme within the gut in inflammatory bowel disease. In ulcerative colitis, strong signals localized to Paneth cell metaplasia were found in 11 of 20 cases and to a lesser degree in non-Paneth cell lineages in regenerative mucosa in 13 of 20 cases. In Crohn's disease, abundant labeling was seen in tuberculoid granulomas (5 of 20) and over macrophage aggregates in the lamina propria in another 7, characteristic patterns not encountered in ulcerative colitis. Low levels of lysozyme messenger RNA were found in the ulceration-associated cell lineage ("pseudopyloric metaplasia"). These results support the view that neutrophils are largely responsible for elevated fecal lysozyme levels in ulcerative colitis and macrophages for elevated serum lysozyme levels in Crohn's disease.
核糖探针原位杂交（rISH）显示溃疡性结肠炎和克罗恩病中存在活性溶菌酶合成。在潘氏细胞、巨噬细胞和肉芽肿中可见最大程度的标记。富含溶菌酶的多形核白细胞对黏膜的弥漫性浸润是溃疡性结肠炎的特征，但在免疫组织化学研究中掩盖了其他细胞谱系的反应性；在多形核白细胞中未检测到溶菌酶mRNA，rISH比免疫组织化学研究更清楚地显示了炎症性肠病中肠道内溶菌酶的活性合成情况。在溃疡性结肠炎中，20例中有11例发现强烈信号定位于潘氏细胞化生，20例中有13例在再生黏膜的非潘氏细胞谱系中信号较弱。在克罗恩病中，20例中有5例在结核样肉芽肿中可见丰富标记，另外7例在固有层的巨噬细胞聚集体上可见丰富标记，这些特征性模式在溃疡性结肠炎中未见到。在溃疡相关细胞谱系（“假幽门化生”）中发现低水平的溶菌酶信使RNA。这些结果支持以下观点，即中性粒细胞在很大程度上导致溃疡性结肠炎患者粪便溶菌酶水平升高，而巨噬细胞导致克罗恩病患者血清溶菌酶水平升高。
