Kontakou M, Sturgess R P, Przemioslo R T, Limb G A, Nelufer J M, Ciclitira P J
Rayne Institute, St Thomas's Hospital, London.
Gut. 1994 Aug;35(8):1037-41. doi: 10.1136/gut.35.8.1037.
In situ hybridisation has been used to study interferon gamma (IFN gamma) mRNA expression in the small intestine of patients with coeliac disease. Sections of jejunal biopsies were obtained from five patients with treated and five with untreated coeliac disease and five disease controls. These sections were hybridised with radiolabelled specific DNA oligonucleotide probes. The lamina propria of untreated coeliac disease patients contained a significantly increased number of IFN gamma producing cells compared with controls but there was no significant difference between the coeliac patients treated with a gluten free diet and controls. The results suggest that IFN gamma may play a part in the immunopathogenesis of coeliac disease.
原位杂交已被用于研究乳糜泻患者小肠中干扰素γ（IFNγ）mRNA的表达。从五名接受治疗的乳糜泻患者、五名未接受治疗的乳糜泻患者以及五名疾病对照者身上获取空肠活检切片。这些切片与放射性标记的特异性DNA寡核苷酸探针进行杂交。与对照组相比，未接受治疗的乳糜泻患者固有层中产生IFNγ的细胞数量显著增加，但采用无麸质饮食治疗的乳糜泻患者与对照组之间无显著差异。结果表明，IFNγ可能在乳糜泻的免疫发病机制中起作用。
