Mitsuma T, Hirooka Y, Kayama M, Mori Y, Adacht K, Takagi J, Wago T, Ping J, Nogimori T
Fourth Department of Internal Medicine, Aichi Medical University, Nagakute, Japan.
Exp Clin Endocrinol Diabetes. 1998;106(2):140-2. doi: 10.1055/s-0029-1211966.
The effect of thyrotropin-releasing hormone (TRH), somatostatin (SS) or octreotide, an analogue of SS, on release of TRH or SS from the rat retina was studied in vitro. The retina was incubated in medium 199 (pH 7.4) with 1.0 mg/ml of bacitracin (medium) for 20 min. The amount of TRH or SS release into the medium was measured by individual radioimmunoassays. The TRH release from the rat retina was inhibited significantly in a dose-related manner by the addition of SS or octreotide. The SS release from the retina was inhibited by TRH, and the inhibitory effect of TRH on SS release from the rat retina was blocked by the addition of anti-TRH receptor antiserum immunoglobulin fraction. The findings suggest an interaction between TRH and SS in the rat retina by which the addition of one inhibits the release of the other.
在体外研究了促甲状腺激素释放激素（TRH）、生长抑素（SS）或SS类似物奥曲肽对大鼠视网膜TRH或SS释放的影响。将视网膜在含有1.0mg/ml杆菌肽的199培养基（pH 7.4）中孵育20分钟。通过个体放射免疫测定法测量释放到培养基中的TRH或SS的量。添加SS或奥曲肽可显著抑制大鼠视网膜TRH的释放，且呈剂量相关方式。TRH可抑制视网膜SS的释放，并且添加抗TRH受体抗血清免疫球蛋白部分可阻断TRH对大鼠视网膜SS释放的抑制作用。这些发现表明大鼠视网膜中TRH与SS之间存在相互作用，即添加一种物质会抑制另一种物质的释放。
