γ-丁酸对体外培养的大鼠视网膜促甲状腺激素释放激素释放的影响。

Effects of gamma-butyric acid on the release of thyrotropin-releasing hormone from the rat retina in vitro.

作者信息

Mitsuma T, Rhue N, Kayama M, Yokoi Y, Izumi M, Adachi K, Wago T, Hirooka Y, Nogimori T

机构信息

Fourth Department of Internal Medicine, Aichi Medical University, Japan.

出版信息

Horm Metab Res. 1997 Sep;29(9):427-9. doi: 10.1055/s-2007-979070.

DOI:10.1055/s-2007-979070

PMID:9370109

Abstract

Effects of gamma-butyric acid (GABA) on the release of thyrotropin-releasing hormone (TRH) from the rat retina in vitro were studied. The rat retina was incubated in medium 199 (pH 7.4) with 1.0 mg/ml of bacitracin and 100 micrograms/ml of ascorbic acid (medium). The amount of TRH release into the medium was measured by radioimmunoassay. The TRH release from the rat retina was inhibited significantly in a dose-related manner with the addition of GABA, but not with bicuculline. The inhibitory effect of GABA on TRH release from the retina was blocked by adding bicuculline to the medium. The findings suggest that the GABAergic system inhibits TRH release from the rat retina in vitro.

摘要

研究了γ-氨基丁酸（GABA）对体外培养的大鼠视网膜促甲状腺激素释放激素（TRH）释放的影响。将大鼠视网膜置于含有1.0mg/ml杆菌肽和100μg/ml抗坏血酸的199培养基（pH 7.4）中（培养基）。通过放射免疫测定法测量释放到培养基中的TRH量。添加GABA后，大鼠视网膜TRH的释放呈剂量依赖性显著抑制，而添加荷包牡丹碱则无此作用。向培养基中添加荷包牡丹碱可阻断GABA对视网膜TRH释放的抑制作用。这些发现表明，体外培养时，GABA能系统抑制大鼠视网膜TRH的释放。