Departments of Civil Engineering and Microbiology, University of New Hampshire, Durham, New Hampshire 03824.
Appl Environ Microbiol. 1983 May;45(5):1659-69. doi: 10.1128/aem.45.5.1659-1669.1983.
The biofilms growing in the first compartments of two rotating biological contactors used to treat municipal wastewater were examined by light and electron microscopy. The biofilms were found to contain a complex and varied microbial community that included filamentous and unicellular bacteria, protozoa, metazoa, and (possibly) bacteriophage. The predominant microorganism among these appeared to be a filamentous bacterium that was identical to Sphaerotilus in both morphological and ultrastructural characteristics. It was possible to isolate a Sphaerotilus-like bacterium from each contactor. Both the Sphaerotilus filaments and the wide variety of unicellular bacteria present tended to contain poly-beta-hydroxybutyrate inclusions, a probable indication that these organisms were removing carbon from the wastewater and storing it. The microbial population of the biofilms appeared to be metabolically active, as evidenced by the presence of microcolonies and dividing cells.
通过光学和电子显微镜检查了用于处理城市废水的两个旋转生物接触器的第一个隔室中生长的生物膜。发现生物膜含有复杂多样的微生物群落，包括丝状和单细胞细菌、原生动物、后生动物和（可能）噬菌体。这些微生物中占优势的似乎是一种丝状细菌，其形态和超微结构特征与鞘丝藻完全相同。可以从每个接触器中分离出一种类似于鞘丝藻的细菌。丝状鞘丝藻和存在的各种单细胞细菌都倾向于含有聚-β-羟基丁酸颗粒，这可能表明这些生物正在从废水中去除碳并将其储存起来。生物膜的微生物种群似乎具有代谢活性，这可以从微菌落和分裂细胞的存在得到证明。
