obligate 甲烷营养菌 Methylosinus trichosporium 菌株 OB3b 的基因组序列。
Genome sequence of the obligate methanotroph Methylosinus trichosporium strain OB3b.
机构信息
Department of Biological Sciences, University of Alberta, CW405 Biological Sciences Centre, Edmonton, Alberta T6G 2E9, Canada.
出版信息
J Bacteriol. 2010 Dec;192(24):6497-8. doi: 10.1128/JB.01144-10. Epub 2010 Oct 15.
Abstract
Methylosinus trichosporium OB3b (for "oddball" strain 3b) is an obligate aerobic methane-oxidizing alphaproteobacterium that was originally isolated in 1970 by Roger Whittenbury and colleagues. This strain has since been used extensively to elucidate the structure and function of several key enzymes of methane oxidation, including both particulate and soluble methane monooxygenase (sMMO) and the extracellular copper chelator methanobactin. In particular, the catalytic properties of soluble methane monooxygenase from M. trichosporium OB3b have been well characterized in context with biodegradation of recalcitrant hydrocarbons, such as trichloroethylene. The sequence of the M. trichosporium OB3b genome is the first reported from a member of the Methylocystaceae family in the order Rhizobiales.
摘要
产甲烷杆菌 OB3b（“奇异”菌株 3b）是一种严格需氧的甲烷氧化α变形菌，最初于 1970 年由罗杰·惠滕伯里（Roger Whittenbury）及其同事分离得到。此后，该菌株被广泛用于阐明甲烷氧化的几个关键酶的结构和功能，包括颗粒态和可溶性甲烷单加氧酶（sMMO）以及细胞外铜螯合剂甲烷菌素。特别是，产甲烷杆菌 OB3b 可溶性甲烷单加氧酶的催化特性在难降解烃类（如三氯乙烯）的生物降解方面得到了很好的描述。产甲烷杆菌 OB3b 基因组序列是第一个在根瘤菌目 Rhizobiales 中的甲基球菌科（Methylocystaceae）成员中报道的序列。
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