Department of Microbiology, Stocking Hall, Cornell University, Ithaca, New York 14853.
Appl Environ Microbiol. 1984 Jun;47(6):1343-5. doi: 10.1128/aem.47.6.1343-1345.1984.
The effects of 2-bromoethanesulfonate, an inhibitor of methanogenesis, on metabolism in sludge from a thermophilic (58 degrees C) anaerobic digestor were studied. It was found from short-term experiments that 1 mumol of 2-bromoethanesulfonate per ml completely inhibited methanogenesis from CH(3)COO, whereas 50 mumol/ml was required for complete inhibition of CO(2) reduction. When 1 mumol of 2-bromoethanesulfonate per ml was added to actively metabolizing sludge which was then incubated for 24 h. it caused a 60% reduction in methanogenesis and a corresponding increase in acetate accumulation; at 50 mumol/ml it caused complete inhibition of methanogenesis and accumulation of acetate. H(2), and ethanol.
研究了 2-溴乙磺酸盐(一种甲烷生成抑制剂)对 58°C 高温厌氧消化器中污泥代谢的影响。通过短期实验发现,每毫升 1 微摩尔的 2-溴乙磺酸盐完全抑制了 CH(3)COO 的甲烷生成,而完全抑制 CO(2)还原则需要 50 微摩尔/毫升。当每毫升 1 微摩尔的 2-溴乙磺酸盐添加到正在代谢的活性污泥中,并孵育 24 小时后,它导致甲烷生成减少 60%,同时乙酸积累增加;当浓度为 50 微摩尔/毫升时,它完全抑制了甲烷生成并导致乙酸、氢气和乙醇的积累。