Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706.
Appl Environ Microbiol. 1980 Jan;39(1):194-204. doi: 10.1128/aem.39.1.194-204.1980.
Anoxic sediments and digested sewage sludge anaerobically oxidized methane to carbon dioxide while producing methane. This strictly anaerobic process showed a temperature optimum between 25 and 37 degrees C, indicating an active microbial participation in this reaction. Methane oxidation in these anaerobic habitats was inhibited by oxygen. The rate of the oxidation followed the rate of methane production. The observed anoxic methane oxidation in Lake Mendota and digested sewage sludge was more sensitive to 2-bromoethanesulfonic acid than the simultaneous methane formation. Sulfate diminished methane formation as well as methane oxidation. However, in the presence of iron and sulfate the ratio of methane oxidized to methane formed increased markedly. Manganese dioxide and higher partial pressures of methane also stimulated the oxidation. The rate of methane oxidation in untreated samples was approximately 2% of the CH(4) production rate in Lake Mendota sediments and 8% of that in digested sludge. This percentage could be increased up to 90% in sludge in the presence of 10 mM ferrous sulfate and at a partial pressure of methane of 20 atm (2,027 kPa).
缺氧沉积物和消化污水污泥在厌氧条件下将甲烷氧化为二氧化碳，同时产生甲烷。这个严格的厌氧过程在 25 到 37 摄氏度之间表现出最佳温度，表明微生物在此反应中起到了积极的作用。这些厌氧环境中的甲烷氧化受到氧气的抑制。氧化的速度与甲烷的产生速度一致。在门多塔湖和消化污水污泥中观察到的缺氧甲烷氧化对 2-溴乙磺酸盐的敏感性高于同时发生的甲烷形成。硫酸盐既减少了甲烷的形成，也减少了甲烷的氧化。然而，在铁和硫酸盐存在的情况下，甲烷被氧化与甲烷形成的比例显著增加。二氧化锰和更高的甲烷分压也刺激了氧化。未经处理的样品中甲烷氧化的速度大约是门多塔湖沉积物中甲烷产生速度的 2%，是消化污泥中甲烷产生速度的 8%。在 10 mM 硫酸亚铁和 20 大气压（2027 kPa）的甲烷分压存在下，污泥中的这一比例可增加到 90%。
