质粒pCU1上杀死肺炎克雷伯菌所需的区域。
Regions on plasmid pCU1 required for the killing of Klebsiella pneumoniae.
作者信息
Thatte V, Gill S, Iyer V N
出版信息
J Bacteriol. 1985 Sep;163(3):1296-9. doi: 10.1128/jb.163.3.1296-1299.1985.
Abstract
Plasmid pCU1 was Kik+ (promotes killing of Klebsiella pneumoniae). All Tn5 insertions within the tra region of pCU1 were Kik-. Two other regions, kikA and kikB, were needed. They may be separated on different plasmids, but both must be mobilized into Klebsiella pneumoniae. Establishment of one kik region in K. pneumoniae followed by receipt of the second did not lead to killing. Kik was therefore intracellular and required concerted and transient action of both regions.
摘要
质粒pCU1是Kik+（促进肺炎克雷伯菌的杀伤）。pCU1转移区（tra region）内的所有Tn5插入突变体都是Kik-。还需要另外两个区域，即kikA和kikB。它们可能位于不同的质粒上，但两者都必须转移到肺炎克雷伯菌中。在肺炎克雷伯菌中先建立一个kik区域，随后再获得第二个区域，并不会导致杀伤作用。因此，Kik作用于细胞内，并且需要两个区域协同且短暂地发挥作用。
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