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真菌中环肽的生物合成及相关序列差异

Cyclodepsipeptide Biosynthesis in Fungi and Sequence Divergence of The .

作者信息

Urbaniak Monika, Waśkiewicz Agnieszka, Trzebny Artur, Koczyk Grzegorz, Stępień Łukasz

机构信息

Plant-Pathogen Interaction Team, Department of Pathogen Genetics and Plant Resistance, Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland.

Department of Chemistry, Poznań University of Life Sciences, Wojska Polskiego 75, 60-625 Poznań, Poland.

出版信息

Pathogens. 2020 Jul 9;9(7):552. doi: 10.3390/pathogens9070552.

DOI:10.3390/pathogens9070552
PMID:32660015
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7400199/
Abstract

Fungi from the order synthesize a range of toxic non-ribosomal cyclic peptides with antimicrobial, insecticidal and cytotoxic activities. Entomopathogenic , and as well as phytopathogenic spp. are known producers of beauvericins (BEAs), beauvenniatins (BEAEs) or enniatins (ENNs). The compounds are synthesized by beauvericin/enniatin synthase (BEAS/ESYN1), which shows significant sequence divergence among members. We investigated ENN, BEA and BEAE production among entomopathogenic (, , ) and phytopathogenic () fungi; BEA and ENNs were quantified using an LC-MS/MS method. Phylogenetic analysis of partial sequences of putative / amplicons was also made. Nineteen fungal strains were identified based on sequence analysis of amplified ITS and -1α regions. BEA was produced by all investigated fungi, with and being the most efficient producers. ENNs were synthesized mostly by , and . The phylogeny reconstruction suggests that ancestral BEA biosynthesis independently diverged into biosynthesis of other compounds. The divergent positioning of three isolates raises the possibility of parallel acquisition of cyclic depsipeptide synthases in ancient complexes within genus. Different fungi have independently evolved NRPS genes involved in depsipeptide biosynthesis, with functional adaptation towards biosynthesis of overlapping yet diversified metabolite profiles.

摘要

该目真菌能合成一系列具有抗菌、杀虫和细胞毒性活性的有毒非核糖体环肽。昆虫病原真菌球孢白僵菌、布氏白僵菌以及植物病原真菌镰孢菌属的一些种是已知的白僵菌素（BEAs）、白僵尼菌素（BEAEs）或恩镰孢菌素（ENNs）的产生菌。这些化合物由白僵菌素/恩镰孢菌素合酶（BEAS/ESYN1）合成，该合酶在不同成员间表现出显著的序列差异。我们研究了昆虫病原真菌（球孢白僵菌、布氏白僵菌、金龟子绿僵菌）和植物病原真菌（镰孢菌属）中ENN、BEA和BEAE的产生情况；采用液相色谱-串联质谱法对BEA和ENNs进行定量分析。还对假定的/扩增子的部分序列进行了系统发育分析。基于扩增的ITS和-1α区域的序列分析鉴定出19株真菌。所有被研究的真菌都能产生BEA，其中球孢白僵菌和布氏白僵菌是最有效的产生菌。ENN主要由镰孢菌属、金龟子绿僵菌和布氏白僵菌合成。系统发育重建表明，祖先的BEA生物合成独立地分化为其他化合物的生物合成。三个镰孢菌属分离株的不同定位增加了在镰孢菌属古老复合体中平行获得环缩肽合酶的可能性。不同的真菌独立进化出参与环缩肽生物合成的非核糖体肽合成酶基因，并对重叠但多样化的代谢产物谱的生物合成进行功能适应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889b/7400199/1e538391fec5/pathogens-09-00552-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889b/7400199/881708623b61/pathogens-09-00552-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889b/7400199/97382b6c66f8/pathogens-09-00552-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889b/7400199/b7f437614ff6/pathogens-09-00552-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889b/7400199/4abcdbd972db/pathogens-09-00552-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889b/7400199/cf8ba8975458/pathogens-09-00552-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889b/7400199/8d4c5a4bff22/pathogens-09-00552-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889b/7400199/fb86d44954c2/pathogens-09-00552-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889b/7400199/1e538391fec5/pathogens-09-00552-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889b/7400199/881708623b61/pathogens-09-00552-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889b/7400199/97382b6c66f8/pathogens-09-00552-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889b/7400199/b7f437614ff6/pathogens-09-00552-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889b/7400199/4abcdbd972db/pathogens-09-00552-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889b/7400199/cf8ba8975458/pathogens-09-00552-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889b/7400199/8d4c5a4bff22/pathogens-09-00552-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889b/7400199/fb86d44954c2/pathogens-09-00552-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889b/7400199/1e538391fec5/pathogens-09-00552-g008.jpg

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