利用嗜热脱硫梭菌连续生产热稳定β-淀粉酶：培养条件和代谢物水平对酶合成和活性的影响。
Continuous Production of Thermostable beta-Amylase with Clostridium thermosulfurogenes: Effect of Culture Conditions and Metabolite Levels on Enzyme Synthesis and Activity.
机构信息
Michigan Biotechnology Institute, 3900 Collins Road, Lansing, Michigan 48909, and Departments of Biochemistry and Microbiology, Michigan State University, East Lansing, Michigan 48824.
出版信息
Appl Environ Microbiol. 1989 Mar;55(3):689-94. doi: 10.1128/aem.55.3.689-694.1989.
Abstract
A beta-amylase-overproducing mutant of Clostridium thermosulfurogenes was grown in continuous culture on soluble starch to produce thermostable beta-amylase. Enzyme productivity was reasonably stable over periods of weeks to months. The pH and temperature optima for beta-amylase production were pH 6.0 and 60 degrees C, respectively. Enzyme concentration was maximized by increasing biomass concentration by using high substrate concentrations and by maintaining a low growth rate. beta-Amylase concentration reached 90 U ml at a dilution rate of 0.07 h in a 3% starch medium. A further increase in enzyme activity levels was limited by acetic acid inhibition of growth and low beta-amylase productivity at low growth rates.
摘要
一株产β-淀粉酶的高温梭菌突变株在连续培养条件下，以可溶性淀粉为底物生产热稳定的β-淀粉酶。在数周到数月的时间内，酶的产率保持相对稳定。β-淀粉酶生产的最适 pH 值和温度分别为 6.0 和 60°C。通过使用高底物浓度和保持低生长速率来增加生物量浓度，从而使酶浓度最大化。在 3%淀粉培养基中，稀释率为 0.07 h 时，β-淀粉酶浓度达到 90 U/ml。由于乙酸对生长的抑制作用以及低生长速率下β-淀粉酶产率低，酶活水平的进一步提高受到限制。
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