ENS.BANA, Laboratoire de Microbiologie, Université de Bourgogne, Campus Universitaire Montmuzard, 21000 Dijon, France.
Appl Environ Microbiol. 1989 Mar;55(3):751-3. doi: 10.1128/aem.55.3.751-753.1989.
A new sensitive medium was developed to screen and isolate mutagenic Leuconostoc oenos strains defective in malolactic fermentation. The essential components of the medium included fructose (22 mM), l-malic acid (74.6 mM), bromocresol green (as pH indicator), and cellulose powder. The wild-type colonies turned blue, but defective malolactic colonies gave an acid reaction and remained yellow-green.
一种新的灵敏培养基被开发出来,用于筛选和分离突变性乳球菌(Leuconostoc oenos)菌株,这些菌株在苹果酸-乳酸发酵中存在缺陷。该培养基的基本成分包括果糖(22mM)、L-苹果酸(74.6mM)、溴甲酚绿(作为 pH 指示剂)和纤维素粉。野生型菌落呈蓝色,而缺陷型的苹果酸-乳酸发酵菌落则呈酸性反应,保持黄绿色。