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筛选丧失苹果酸-乳酸发酵能力的片球菌的培养基。

Medium for Screening Leuconostoc oenos Strains Defective in Malolactic Fermentation.

机构信息

ENS.BANA, Laboratoire de Microbiologie, Université de Bourgogne, Campus Universitaire Montmuzard, 21000 Dijon, France.

出版信息

Appl Environ Microbiol. 1989 Mar;55(3):751-3. doi: 10.1128/aem.55.3.751-753.1989.

DOI:10.1128/aem.55.3.751-753.1989
PMID:16347882
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC184191/
Abstract

A new sensitive medium was developed to screen and isolate mutagenic Leuconostoc oenos strains defective in malolactic fermentation. The essential components of the medium included fructose (22 mM), l-malic acid (74.6 mM), bromocresol green (as pH indicator), and cellulose powder. The wild-type colonies turned blue, but defective malolactic colonies gave an acid reaction and remained yellow-green.

摘要

一种新的灵敏培养基被开发出来，用于筛选和分离突变性乳球菌（Leuconostoc oenos）菌株，这些菌株在苹果酸-乳酸发酵中存在缺陷。该培养基的基本成分包括果糖（22mM）、L-苹果酸（74.6mM）、溴甲酚绿（作为 pH 指示剂）和纤维素粉。野生型菌落呈蓝色，而缺陷型的苹果酸-乳酸发酵菌落则呈酸性反应，保持黄绿色。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85fe/184191/ee5d00bb999e/aem00096-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85fe/184191/ee5d00bb999e/aem00096-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85fe/184191/ee5d00bb999e/aem00096-0218-a.jpg

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