Insect Pathology Laboratory, BARC-West, Building 011A, Room 214, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705.
Appl Environ Microbiol. 1989 May;55(5):1049-51. doi: 10.1128/aem.55.5.1049-1051.1989.
We compared the replication of the gypsy moth (Lymantria dispar) nuclear polyhedrosis virus in two new cell lines, from embryos and fat body of L. dispar, and in a previously available ovarian cell line. Three virus isolates (the Hamden strain [LDP-67] used commercially as GYPCHEK, a plaque-purified clone of Hamden [5-7d], and an isolate from Abington, Mass. [Ab]) were each tested on the three cell lines. The fat-body-derived cell line proved best in terms of occlusion body production for all three virus strains, with the highest yield produced by the Abington strain. On the basis of these results, we conclude that a more efficient in vitro production of gypsy moth virus can be obtained by using the fat body cell line in conjunction with the Abington strain of the virus.
我们比较了舞毒蛾（Lymantria dispar）核多角体病毒在两种新细胞系中的复制情况，这两种细胞系分别来自舞毒蛾的胚胎和脂肪体，以及之前可用的卵巢细胞系。三种病毒分离株（商业上用作 GYPCHEK 的哈姆登株[LDP-67]、哈姆登的斑块纯化克隆[5-7d]和马萨诸塞州阿宾顿的分离株[Ab]）分别在这三种细胞系上进行了测试。脂肪体衍生的细胞系在三种病毒株的所有方面都证明了最好的包含体生产，其中阿宾顿株产生的产量最高。基于这些结果，我们得出结论，通过使用脂肪体细胞系和阿宾顿株病毒，可以更有效地在体外生产舞毒蛾病毒。
