Appl Environ Microbiol. 1996 Jun;62(6):2186-90. doi: 10.1128/aem.62.6.2186-2190.1996.
A diverse collection of actinomycete strains were screened for production of extracellular peroxidase activity by adapting a chemiluminescence analysis system developed for horseradish peroxidase-based enzyme-linked immunosorbent assay. Extracellular peroxidase activity was found to be common but quantitatively variable, and this rapid and sensitive screening system permitted identification of a small group of high-producing strains. A range of spectrophotometric assays were compared for the measurement of peroxidase activity in concentrated culture supernatants of two selected thermophilic streptomycetes. Of these, the peroxide-dependent oxidation of 2,4-dichlorophenol was identified as the most robust and reproducible assay for quantitative studies.
采用一种改良的辣根过氧化物酶酶联免疫吸附测定化学发光分析系统，筛选了多种放线菌菌株以产生细胞外过氧化物酶活性。发现细胞外过氧化物酶活性普遍存在，但数量上有所不同，这种快速灵敏的筛选系统可以鉴定出一小部分高产菌株。比较了一系列分光光度法测定两种选定的嗜热链霉菌浓缩培养上清液中过氧化物酶活性的方法。其中，过氧化物依赖性 2,4-二氯苯酚氧化被确定为定量研究最稳健和可重复的测定方法。
