Department of Chemical Engineering, Laboratory of Biochemistry and Microbiology, Helsinki University of Technology, SF-02150 Espoo, Finland.
Appl Environ Microbiol. 1992 May;58(5):1656-60. doi: 10.1128/aem.58.5.1656-1660.1992.
The fatty acid composition of Thermus spp., including T. aquaticus ATCC 25104, T. thermophilus DSM 579, T. flavus DSM 674, and seven wild strains was examined. Organisms were tested at a minimum of either 35, 40, or 45 degrees C and at an optimum of 60 or 70 degrees C. Total fatty acid content per dry weight of cells varied between 1.2 and 3.7%, and the quantity of fatty acids was higher at the high temperature range in the majority of strains. At the optimum temperature, strains could be assigned to three chemotaxonomic groups with reference to the ratio of iso C(15:0)/iso C(17:0). In six of the strains the ratio of iso C(15:0)/iso C(17:0) remained unchanged at the minimum temperature, whereas in four strains the ratio was reversed. The proportion of the C(15:0) and C(17:0) isobranched acids was decreased and the proportion of anteisobranched fatty acids, namely anteiso C(15:0), anteiso C(17:0), and anteiso C(17:1), was increased at the lower temperature range. Some changes were seen in the levels of the n-C(16:0) and iso C(16:0) acids, but these were strain specific.
研究了嗜热菌属（包括水生栖热菌 ATCC 25104、嗜热脂肪芽孢杆菌 DSM 579、黄色栖热菌 DSM 674 以及 7 株野生菌株）的脂肪酸组成。在最低 35、40 或 45°C 以及最佳 60 或 70°C 下对生物体进行了测试。细胞干重的总脂肪酸含量在 1.2%至 3.7%之间变化，并且在大多数菌株的高温范围内，脂肪酸的数量较高。在最佳温度下，根据 iso C(15:0)/iso C(17:0)的比例，可以将菌株分为三个化学分类群。在 6 株菌株中，iso C(15:0)/iso C(17:0)的比例在最低温度下保持不变，而在 4 株菌株中则发生了反转。C(15:0)和 C(17:0)异支链酸的比例降低，而 anteiso C(15:0)、anteiso C(17:0)和 anteiso C(17:1)等 anteiso 支链脂肪酸的比例在较低温度范围内增加。n-C(16:0)和 iso C(16:0)酸的水平也发生了一些变化，但这是菌株特异性的。
