Castle A J, Horgen P A, Anderson J B
Appl Environ Microbiol. 1987 Apr;53(4):816-22. doi: 10.1128/aem.53.4.816-822.1987.
Two Agaricus species, A. brunnescens (a commercial mushroom) and A. bitorquis (a wild, edible species), were examined for restriction fragment length polymorphisms. EcoRI-digested nuclear DNA from isolates of both species were cloned in plasmid vector pUC18. Ten random recombinant clones were used in Southern DNA-DNA hybridizations to probe EcoRI-digested DNA from 11 A. brunnescens isolates (7 commercial, 2 wild type, and 2 homokaryotic) and 7 A. bitorquis isolates. Most cloned fragments were polymorphic in both species. There were fewer different genotypes than expected, however, in the sample of commercial A. brunnescens strains. DNA from homokaryotic strains showed fewer bands in most hybridizations than DNA from heterokaryotic strains. All A. bitorquis isolates could be distinguished from each other as well as from every A. brunnescens strain. Putative homokaryons were detected by the loss of polymorphic bands among protoplast regenerates from one commercial strain and two strains collected in the wild.
对两种姬松茸，即双孢蘑菇（一种商业栽培蘑菇）和大肥菇（一种野生可食用品种）进行了限制性片段长度多态性分析。用EcoRI酶切两种菌株分离物的核DNA，并克隆到质粒载体pUC18中。从十个随机重组克隆中提取的DNA用于Southern DNA-DNA杂交，以探测用EcoRI酶切的来自11个双孢蘑菇分离物（7个商业菌株、2个野生型和2个同核体）以及7个大肥菇分离物的DNA。大多数克隆片段在两种菌株中均具有多态性。然而，在商业双孢蘑菇菌株样本中，不同基因型的数量比预期的少。在大多数杂交中，同核体菌株的DNA条带比异核体菌株的少。所有大肥菇分离物彼此之间以及与每个双孢蘑菇菌株都能区分开来。从一个商业菌株和两个野生采集菌株的原生质体再生体中多态性条带的缺失检测到了假定的同核体。
