Department of Microbiology, Spaulding Life Science Center, University of New Hampshire, Durham, New Hampshire 03824.
Appl Environ Microbiol. 1994 Nov;60(11):4189-91. doi: 10.1128/aem.60.11.4189-4191.1994.
MilliQ water was inoculated with poliovirus type 1 strain LSc-1 and was treated with disinfectants, including chlorine, chlorine dioxide, ozone, and UV light. No relationship between probes and plaque assays were seen, demonstrating that viral nucleic acids were not destroyed. These findings suggest that nucleic acid probes cannot distinguish between infectious and noninfectious viruses and cannot be used in the evaluation of treated waters.
MilliQ 水接种了脊髓灰质炎病毒 1 型 LSc-1 株,并经过消毒剂处理,包括氯、二氧化氯、臭氧和紫外线。探针和蚀斑测定之间没有关系,表明病毒核酸没有被破坏。这些发现表明,核酸探针不能区分感染性和非感染性病毒,不能用于处理水的评估。