Moore N J, Margolin A B
Department of Microbiology, University of New Hampshire, Durham 03824.
Appl Environ Microbiol. 1993 Sep;59(9):3145-6. doi: 10.1128/aem.59.9.3145-3146.1993.
Five nonradioactive probe assays were evaluated by using chemiluminescent and colormetric signals, along with two isotopic assays and cell culture, for the detection of poliovirus in concentrated water samples. In environmental samples, a 100% correlation existed between digoxigenin and single-stranded [32P]RNA probes. All probe assays detected more positive samples than the cell culture did.
使用化学发光和比色信号，结合两种同位素检测法和细胞培养法，对五种非放射性探针检测法进行了评估，以检测浓缩水样中的脊髓灰质炎病毒。在环境样本中，地高辛配基与单链[32P]RNA探针之间存在100%的相关性。所有探针检测法检测到的阳性样本都比细胞培养法多。
