通过在胰蛋白酶 - 柠檬酸盐溶液中对组织进行低温孵育改进细胞分散方法。
Improved cell dispersal method by low-temperature incubation of tissue in a trypsin-citrate solution.
作者信息
van Wezel A L, van Hemert P A, Parisius W, Cucakovich N B, Macmorine H G
机构信息
Rijks Instituut voor de Volksgezondheid, Postbus 1, Bilthoven, The Netherlands.
出版信息
Appl Microbiol. 1972 Sep;24(3):506-7. doi: 10.1128/am.24.3.506-507.1972.
Abstract
Tissues soaked at low temperature in a trypsin-citrate solution, washed to remove enzymes, and disrupted by rapid mechanical vibrations gave yields of viable cells 1.6 to 5 times those from conventional procedures.
摘要
将组织在低温下浸泡于胰蛋白酶 - 柠檬酸盐溶液中，洗涤以去除酶，然后通过快速机械振动使其破碎，所获得的活细胞产量是传统方法的1.6至5倍。
相似文献
1
Improved cell dispersal method by low-temperature incubation of tissue in a trypsin-citrate solution.通过在胰蛋白酶 - 柠檬酸盐溶液中对组织进行低温孵育改进细胞分散方法。
Appl Microbiol. 1972 Sep;24(3):506-7. doi: 10.1128/am.24.3.506-507.1972.
2
A method for isolating large numbers of viable disaggregated cells from various human tissues for cell culture establishment.
In Vitro Cell Dev Biol. 1986 Sep;22(9):529-34. doi: 10.1007/BF02621139.
3
Cell dispersal methods for increasing yield from animal tissues.用于提高动物组织产量的细胞分散方法。
Appl Microbiol. 1969 Apr;17(4):524-7. doi: 10.1128/am.17.4.524-527.1969.
4
The inactivation of dilute solutions of crystalline trypsin by x-radiation. II. Effects of enzyme concentration, medium, pH, and temperature.X射线辐射对结晶胰蛋白酶稀溶液的失活作用。II. 酶浓度、介质、pH值及温度的影响
J Gen Physiol. 1955 May 20;38(5):581-98. doi: 10.1085/jgp.38.5.581.
5
Effect of chemical treatments on tendon cellularity and mechanical properties.化学处理对肌腱细胞密度和力学性能的影响。
J Biomed Mater Res. 2000 Jan;49(1):134-40. doi: 10.1002/(sici)1097-4636(200001)49:1<134::aid-jbm17>3.0.co;2-d.
6
University of wisconsin solution with trypsin inhibitor pefabloc improves survival of viable human and primate impure islets during storage.含有胰蛋白酶抑制剂苯甲脒的威斯康星大学溶液可提高人及灵长类动物不纯胰岛在储存期间的存活能力。
Cell Tissue Bank. 2001;2(1):15-21. doi: 10.1023/A:1011585929679.
7
Peptic and tryptic hydrolysis of native and heated whey protein to reduce its antigenicity.对天然和加热的乳清蛋白进行胃蛋白酶和胰蛋白酶水解以降低其抗原性。
J Dairy Sci. 2007 Sep;90(9):4043-50. doi: 10.3168/jds.2007-0169.
8
Cell preparation method with trypsin digestion for counting of colony forming units in Candida albicans-infected mucosal tissues.用胰酶消化法制备细胞，用于计算白念珠菌感染黏膜组织中的菌落形成单位。
Med Mycol. 2012 Nov;50(8):858-62. doi: 10.3109/13693786.2012.683538. Epub 2012 May 31.
9
Disaggregation of human solid tumours by combined mechanical and enzymatic methods.通过机械和酶促方法联合对人实体瘤进行解离
Br J Cancer. 1985 Jan;51(1):93-8. doi: 10.1038/bjc.1985.13.
10
Comparative viabilities of rat intestinal epithelial cells prepared by mechanical, enzymatic and chelating methods.通过机械、酶解和螯合方法制备的大鼠肠上皮细胞的比较活力。
Digestion. 1981;21(1):25-32. doi: 10.1159/000198516.
引用本文的文献
1
An immunofluorescence study of influenza virus filament formation.流感病毒丝状结构形成的免疫荧光研究。
Arch Virol. 1980;63(3-4):275-84. doi: 10.1007/BF01315033.
本文引用的文献
1
An apparatus for producing trypsinized monkey kidney cell suspensions.一种用于制备胰蛋白酶处理的猴肾细胞悬液的装置。
Virology. 1960 Feb;10:280-3. doi: 10.1016/0042-6822(60)90048-9.
2
Trypsinization of monkey-kidney tissue: an automatic method for the preparation of cell suspensions.猴肾组织的胰蛋白酶消化：一种制备细胞悬液的自动化方法。
Bull World Health Organ. 1956;14(1):147-66.
3
Plaque formation and isolation of pure lines with poliomyelitis viruses.脊髓灰质炎病毒的噬斑形成及纯系分离
J Exp Med. 1954 Feb;99(2):167-82. doi: 10.1084/jem.99.2.167.
4
Poliomyelitis viruses in tissue culture. IV. Protein-free nutrient media in stationary and roller tube cultures.组织培养中的脊髓灰质炎病毒。IV. 静止和滚管培养中的无蛋白营养培养基。
Proc Soc Exp Biol Med. 1952 Oct;81(1):208-13. doi: 10.3181/00379727-81-19823.
Zotero 插件