Guan Jingjiao, Lee L James
Center for Affordable Nanoengineering of Polymer Biomedical Devices, Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, USA.
Proc Natl Acad Sci U S A. 2005 Dec 20;102(51):18321-5. doi: 10.1073/pnas.0506902102. Epub 2005 Dec 13.
Highly ordered arrays of stretched DNA molecules were generated over the millimeter scale by using a modified molecular combing method and soft lithography. Topological micropatterning on polydimethyl siloxane stamps was used to mediate the dynamic assembly of DNA molecules into arranged nonostrand arrays. These arrays consisted of either short nanostrands of several micrometers with fixed length and orientation or long nanostrands up to several hundred micrometers in length. The nanostrand arrays were transferred onto flat solid surfaces by contact printing, allowing for the creation of more complex patterns. This technique has potential applications for the construction of next-generation DNA chips and functional circuits of DNA-based 1D nanostructures.
通过使用改良的分子梳技术和软光刻技术,在毫米尺度上生成了高度有序排列的拉伸DNA分子阵列。利用聚二甲基硅氧烷印章上的拓扑微图案化来介导DNA分子动态组装成排列有序的单链阵列。这些阵列由长度固定且方向固定的几微米短纳米链或长度达几百微米的长纳米链组成。通过接触印刷将纳米链阵列转移到平坦的固体表面上,从而能够创建更复杂的图案。该技术在构建下一代DNA芯片和基于DNA的一维纳米结构功能电路方面具有潜在应用。