Misra Neelam, Gupta Ajay K
Department of Biochemistry, Bundelkhand University, Jhansi (UP), India.
J Plant Physiol. 2006 Jan;163(1):11-8. doi: 10.1016/j.jplph.2005.02.011. Epub 2005 Aug 10.
The activities of antioxidant enzymes viz. glutathione reductase, GR; superoxide dismutase, SOD; peroxidase, POD; catalase, CAT and glutathione-S-transferase, GST and alkaloid accumulation were investigated in leaf pairs (apical, middle, basal) and in roots of Catharanthus roseus seedlings under the conditions of different nitrogen sources (20 mM KNO(3) and 2 mM NH(4)Cl) and salinity, in the absence (non-saline control) and in the presence of 100 mM NaCl in the nutrient solution. Salinity caused a reduction in plant biomass. The biomass production of ammonium-fed plants was lower than that of nitrate-fed plants. The antioxidant enzymes exhibited higher activity in saline-treated plants. Changes in antioxidant enzyme activity caused by different nitrogen sources differed in all leaf pairs, as well as in roots of C. roseus. Ammonium-fed plants showed higher CAT, GR and GST activity in leaf pairs as well as in roots, while POD and SOD activity were higher in nitrate-fed plants. Higher peroxidase activity concomitant with the increased accumulation of alkaloid was found in all leaf pairs, as well as in roots of C. roseus of NO(3)(-) fed plants as compared to NH(4)(+) fed plants.
在不同氮源（20 mM KNO₃和2 mM NH₄Cl）和盐度条件下，即在营养液中不存在（非盐对照）和存在100 mM NaCl的情况下，研究了长春花幼苗叶对（顶端、中部、基部）和根中抗氧化酶（即谷胱甘肽还原酶，GR；超氧化物歧化酶，SOD；过氧化物酶，POD；过氧化氢酶，CAT和谷胱甘肽 - S - 转移酶，GST）的活性以及生物碱的积累情况。盐度导致植物生物量减少。铵供应植物的生物量产量低于硝酸盐供应植物。抗氧化酶在盐处理植物中表现出更高的活性。不同氮源引起的抗氧化酶活性变化在长春花的所有叶对以及根中均有所不同。铵供应植物在叶对和根中均表现出较高的CAT、GR和GST活性，而硝酸盐供应植物中的POD和SOD活性较高。与铵供应植物相比，在硝酸盐供应的长春花的所有叶对以及根中均发现，过氧化物酶活性较高且生物碱积累增加。
