Studelska D R, Campbell C, Pang S, Rodnick K J, James D E
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110.
Am J Physiol. 1992 Jul;263(1 Pt 1):E102-6. doi: 10.1152/ajpendo.1992.263.1.E102.
The insulin-regulatable glucose transporter (GLUT-4) is expressed in adipose tissue and in cardiac and skeletal muscle (D. E. James, R. Brown, J. Navarro, and P. F. Pilch. Nature Lond. 333: 183-185, 1988). We examined GLUT-4 development between postnatal days 1 and 41 (P1-P41) in male and female rats in these tissues by quantitative immunoblotting. GLUT-4 was detectable in each tissue at comparable levels at P1. However, the subsequent patterns of GLUT-4 development were distinctive. GLUT-4 increased in the diaphragm after P7, peaked at P20, and then declined. GLUT-4 expression in the heart increased rapidly after P7 to plateau on P41 at levels four times greater than the diaphragm. In sharp contrast, adipose tissue expression was highest between P3 and P5 but declined to a nadir at P20 before rebounding at P34. These patterns were observed for both sexes within each tissue, but female GLUT-4 expression was higher in diaphragm and heart and lower in adipose tissue. The expression of GLUT-4 appears to be regulated in a tissue-specific manner by a developmental program that may coordinate the expression of other proteins of metabolic importance.
胰岛素可调节的葡萄糖转运体（GLUT - 4）在脂肪组织、心肌和骨骼肌中表达（D. E. 詹姆斯、R. 布朗、J. 纳瓦罗和P. F. 皮尔希。《自然》伦敦版。333: 183 - 185, 1988）。我们通过定量免疫印迹法研究了出生后第1天至第41天（P1 - P41）雄性和雌性大鼠这些组织中GLUT - 4的发育情况。在P1时，每个组织中都能检测到水平相当的GLUT - 4。然而，随后GLUT - 4的发育模式各不相同。膈肌中的GLUT - 4在P7后增加，在P20达到峰值，然后下降。心脏中GLUT - 4的表达在P7后迅速增加，在P41达到稳定水平，是膈肌中水平的四倍。与之形成鲜明对比的是，脂肪组织中的表达在P3至P5之间最高，但在P20降至最低点，然后在P34反弹。在每个组织的两性中都观察到了这些模式，但雌性GLUT - 4在膈肌和心脏中的表达较高，在脂肪组织中的表达较低。GLUT - 4的表达似乎受一个发育程序以组织特异性方式调节，该程序可能协调其他具有代谢重要性的蛋白质的表达。
