Tessem May-Britt, Midelfart Anna, Cejková Jitka, Bathen Tone F
Department of Neuroscience, Faculty of Medicine, Norwegian University of Science and Technology, NTNU, Trondheim, Norway.
Ophthalmic Res. 2006;38(2):105-14. doi: 10.1159/000090511. Epub 2005 Dec 22.
The aim of the study was to investigate the metabolic profiles of intact rabbit corneas and lenses exposed to UVA and UVB radiation by using high-resolution (HR) magic angle spinning (MAS) (1)H nuclear magnetic resonance (NMR) spectroscopy and pattern recognition methods.
Adult albino rabbits were exposed to UVA (366 nm, 0.589 J/cm(2)) or UVB (312 nm, 1.667 J/cm(2)) radiation for 8 min, once a day for 5 days. Three days after the last irradiation day, samples of corneas and lenses were dissected. HR-MAS (1)H NMR spectroscopy combined with pattern recognition methods (principal component analysis and soft independent modelling of class analogy) and one-way ANOVA were applied to obtain metabolic information from intact corneal and lens tissue.
UVB irradiation caused statistically significant metabolic changes in the rabbit corneas. A decrease in metabolites as ascorbate (84%), myo-inositol (59%), hypotaurine (91%) and choline (76%) was observed. Exposure to UVA radiation caused no significant metabolic alteration in this tissue. The metabolic profile of the rabbit lenses showed no detectable changes after UVA or UVB exposure.
The combination of HR-MAS (1)H NMR spectroscopy and multivariate methods proved effective to analyse intact corneal and lens tissue after exposure to UV radiation of different wavelengths. By avoiding extraction methods and obtaining complete metabolic profiles from one sample, HR-MAS (1)H NMR spectroscopy provided important information about metabolic alteration occurring in rabbit corneal and lens tissue after UV exposure.
本研究旨在通过使用高分辨率（HR）魔角旋转（MAS）氢核磁共振（NMR）光谱和模式识别方法，研究暴露于UVA和UVB辐射下的完整兔角膜和晶状体的代谢谱。
成年白化兔每天接受一次UVA（366 nm，0.589 J/cm²）或UVB（312 nm，1.667 J/cm²）辐射8分钟，持续5天。在最后一次照射后的第三天，解剖角膜和晶状体样本。应用HR-MAS氢核磁共振光谱结合模式识别方法（主成分分析和类类比软独立建模）以及单因素方差分析，从完整的角膜和晶状体组织中获取代谢信息。
UVB辐射导致兔角膜出现具有统计学意义的代谢变化。观察到抗坏血酸（84%）、肌醇（59%）、亚牛磺酸（91%）和胆碱（76%）等代谢物减少。暴露于UVA辐射未引起该组织明显的代谢改变。兔晶状体的代谢谱在UVA或UVB暴露后未显示出可检测到的变化。
HR-MAS氢核磁共振光谱与多变量方法相结合，被证明在分析不同波长紫外线照射后的完整角膜和晶状体组织方面是有效的。通过避免提取方法并从一个样本中获得完整的代谢谱，HR-MAS氢核磁共振光谱提供了有关兔角膜和晶状体组织在紫外线暴露后发生的代谢改变的重要信息。
