Rouse B T, Babiuk L A
Infect Immun. 1975 Mar;11(3):505-11. doi: 10.1128/iai.11.3.505-511.1975.
An in vitro method is described to measure the inhibitory activity of murine peritoneal exudate cells against viral plaque formation by a bovine herpes-virus-infectious bovin'e rhinotracheitis virus. Microtiter plates containing 96 bovine kidney cell monolayers were infected with a range of virus concentration and peritoneal exudate cells were subsequently added. When a sufficient number of cells was added, viral plaques were not detectable and free infectious virus did not occur in the culture fluids. The inhibitory cell type adhered to glass and was presumably a macrohage. Although inhibitory of viral plaques was presumably a macrophage. Although inhibition of viral plaques was complete and free virus could not be detected, virus was not eliminated from the monolayers since on removal of cells, the degree of virus cytopathology and yield of virus after a further 48 h of incubation was the same as in 48-h infected control monolayers. The significance of peritoneal exudate-cells-induced virus suppression as a model to understand herpesvirus latency is briefly discussed.?Author
本文描述了一种体外方法，用于测量小鼠腹腔渗出细胞对牛疱疹病毒（传染性牛鼻气管炎病毒）形成病毒蚀斑的抑制活性。将含有96个牛肾细胞单层的微量滴定板用一系列病毒浓度进行感染，随后加入腹腔渗出细胞。当加入足够数量的细胞时，病毒蚀斑无法检测到，并且培养液中也不会出现游离的感染性病毒。具有抑制作用的细胞类型附着于玻璃，推测为巨噬细胞。虽然抑制病毒蚀斑的可能是巨噬细胞。尽管对病毒蚀斑的抑制是完全的，且无法检测到游离病毒，但病毒并未从单层细胞中消除，因为在去除细胞后，再经过48小时培养，病毒细胞病变程度和病毒产量与感染48小时的对照单层细胞相同。本文简要讨论了腹腔渗出细胞诱导的病毒抑制作为理解疱疹病毒潜伏模型的意义。?作者
