Rawlins J M, Lam W L, Karoo R O, Naylor I L, Sharpe D T
Plastic Surgery Department, Castle Hill Hospital, Cottingham, Hull HU16 5JQ, UK.
Burns. 2006 Feb;32(1):42-5. doi: 10.1016/j.burns.2005.08.004. Epub 2005 Dec 27.
Fibroblasts are thought to be (in part) responsible for the persisting contractile forces that result in burn contractures. Using monolayer and fibroblast populated collagen lattice (FPCL) models we subjected burn scar fibroblasts to the anti-fibrinolytic agent Pentoxifylline (PFX) in an attempt to reduce proliferation and contraction of these cells. Fibroblasts were isolated from mature burn scars at reconstructive surgery. Fibroblasts were grown in monolayer or incorporated into FPCL's and exposed to PFX. Fibroblast numbers and FPCL surface areas were calculated using digital photography and image analysis. PFX showed a dose-dependent inhibition of contraction and reduced proliferation of burn scar fibroblasts. In monolayer, cell number proliferation was markedly reduced. FPCL's containing 0, 0.25, 0.5, 1, and 2 mg/ml of PFX had relative surface areas of 31, 40, 43, 59, and 85%, respectively. One and 2 mg/ml FPCL's contracted significantly less than controls (p < 0.0001). This is the first study to show the dose-dependent effects of Pentoxifylline on the proliferation and contraction of burn scar fibroblasts. This study suggests that Pentoxifylline has a direct effect on inhibiting burn scar fibroblasts. Further study of PFX on burn scars will provide opportunities to reduce burn scar contractures in vivo.
成纤维细胞被认为（部分）是导致烧伤挛缩的持续收缩力的原因。我们使用单层和成纤维细胞填充胶原晶格（FPCL）模型，将烧伤瘢痕成纤维细胞置于抗纤溶药物己酮可可碱（PFX）中，试图减少这些细胞的增殖和收缩。在重建手术中从成熟烧伤瘢痕中分离出成纤维细胞。将成纤维细胞培养成单层或整合到FPCL中，并暴露于PFX。使用数码摄影和图像分析计算成纤维细胞数量和FPCL表面积。PFX对烧伤瘢痕成纤维细胞的收缩具有剂量依赖性抑制作用，并减少其增殖。在单层培养中，细胞数量增殖明显减少。含有0、0.25、0.5、1和2mg/ml PFX的FPCL的相对表面积分别为31%、40%、43%、59%和85%。1mg/ml和2mg/ml的FPCL收缩明显小于对照组（p<0.0001）。这是第一项显示己酮可可碱对烧伤瘢痕成纤维细胞增殖和收缩具有剂量依赖性影响的研究。该研究表明己酮可可碱对抑制烧伤瘢痕成纤维细胞有直接作用。对PFX在烧伤瘢痕上的进一步研究将为减少体内烧伤瘢痕挛缩提供机会。
