Kaneko Yasuko, Danev Radostin, Nagayama Kuniaki, Nakamoto Hitoshi
Department of Biochemistry and Molecular Biology, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama 338-8570, Japan.
J Bacteriol. 2006 Jan;188(2):805-8. doi: 10.1128/JB.188.2.805-808.2006.
Carboxysomes in rapidly frozen ice-embedded whole cells of the cyanobacterium Synechococcus sp. strain PCC 7942 were visualized by the recently developed Hilbert differential contrast transmission electron microscope. Structural details of carboxysomes were especially clearly visualized in the ruptured cells. The novel electron microscopy exhibited the paracrystalline arrays of molecules of the enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase in the carboxysomes in much better contrast than conventional transmission electron microscopy with ultrathin sections of cells. The carboxysome was surrounded by a 5- to 6-nm-thick monolayer shell which consisted of orderly arrays of globular particles.
利用最近开发的希尔伯特微分相衬透射电子显微镜，对蓝藻聚球藻属PCC 7942菌株快速冷冻、冰包埋的全细胞中的羧酶体进行了观察。在破裂的细胞中，羧酶体的结构细节尤为清晰可见。这种新型电子显微镜显示，羧酶体中1,5-二磷酸核酮糖羧化酶/加氧酶分子的准晶体阵列，其对比度比传统的细胞超薄切片透射电子显微镜要好得多。羧酶体被一层5至6纳米厚的单层外壳包围，该外壳由排列有序的球状颗粒组成。
