人乳头多瘤空泡病毒BK的病毒粒子多肽组成：与猴病毒40和多瘤病毒的比较
Virion polypeptide composition of the human papovavirus BK: comparison with simian virus 40 and polyoma virus.
作者信息
Wright P J, Di Mayorca G
出版信息
J Virol. 1975 Apr;15(4):828-35. doi: 10.1128/JVI.15.4.828-835.1975.
The polypeptide composition of labeled BK virus was compared with that of simian virus 40 (SV40) and polyoma virus by co-electrophoresis of disrupted virions in polyacrylamide gels containing approximately 73% of the capsid protein and had a molecular weight of 39,000. It was smaller than VP1 of SV40 and polyoma virus. The other polypeptides of BK virus were similar in molecular weight to those of SV40. A comparison of the proteins of BK virus and SV40 iodinated with chloramine T before and after disruption in alkaline buffer at pH 10.5 revealed differences between the two viruses in the number and distribution of tyrosines available for iodination. The tryptic peptides of VP1, VP3, VP4, and VP5 combined of SV40 were compared with those of the same polypeptides of BK virus. Among the 19 peptides of VP1 resolved, only two were common to both viruses. The analyses of VP4 and VP5, the histone-like proteins, however, showed more similarity between the viruses, with 6 of 15 resolved peptides in common. The tryptic digests of VP3 were completely different.
通过在含有约73%衣壳蛋白且分子量为39,000的聚丙烯酰胺凝胶中对裂解的病毒粒子进行共电泳，将标记的BK病毒的多肽组成与猴病毒40（SV40）和多瘤病毒的多肽组成进行了比较。它比SV40和多瘤病毒的VP1小。BK病毒的其他多肽分子量与SV40的相似。对BK病毒和SV40在pH 10.5的碱性缓冲液中裂解前后用氯胺T碘化的蛋白质进行比较，发现两种病毒在可用于碘化的酪氨酸数量和分布上存在差异。比较了SV40的VP1、VP3、VP4和VP5组合的胰蛋白酶肽与BK病毒相同多肽的胰蛋白酶肽。在VP1的19个肽中，两种病毒只有两个是共同的。然而，对组蛋白样蛋白VP4和VP5的分析表明，两种病毒之间的相似性更高，在15个解析肽中有6个是共同的。VP3的胰蛋白酶消化产物完全不同。
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