Mason W S, Aldrich C, Summers J, Taylor J M
Proc Natl Acad Sci U S A. 1982 Jul;79(13):3997-4001. doi: 10.1073/pnas.79.13.3997.
In order to study the replication of the DNA genome of duck hepatitis B virus, an avian virus related to human hepatitis B virus, we have characterized viral DNAs present in the livers of viremic ducks by agarose gel electrophoresis and the Southern blot procedure. In addition to relaxed circular DNA similar to virion DNA, livers contained a heterogeneous population of rapidly migrating species. The conformation of the rapidly migrating species was markedly sensitive to salt, suggesting that these species were largely single stranded. The largest major rapidly migrating species was shown to have an electrophoretic mobility that was insensitive to preheating of the DNA to 100 degrees C and was similar to that of denatured virus DNA 3 kilobases long, suggesting that this DNA was a single-stranded copy of the entire virus genome. Hybridization with strand-specific probes demonstrated that this 3-kilobase species, as well as more rapidly migrating DNAs, were predominantly minus strands.
为了研究与人类乙型肝炎病毒相关的禽病毒——鸭乙型肝炎病毒DNA基因组的复制，我们通过琼脂糖凝胶电泳和Southern印迹法对病毒血症鸭肝脏中存在的病毒DNA进行了表征。除了类似于病毒粒子DNA的松弛环状DNA外，肝脏中还含有一群快速迁移的异质物种。快速迁移物种的构象对盐非常敏感，这表明这些物种在很大程度上是单链的。最大的主要快速迁移物种显示出其电泳迁移率对DNA预热至100摄氏度不敏感，并且与3千碱基长的变性病毒DNA相似，这表明该DNA是整个病毒基因组的单链拷贝。与链特异性探针杂交表明，这个3千碱基的物种以及迁移更快的DNA主要是负链。
