将兔β-珠蛋白基因显微注射到受精卵中及其在成年小鼠及其后代中的后续表达。

Wagner T E, Hoppe P C, Jollick J D, Scholl D R, Hodinka R L, Gault J B

Proc Natl Acad Sci U S A. 1981 Oct;78(10):6376-80. doi: 10.1073/pnas.78.10.6376.

We have transferred a gene coding for rabbit beta-globin into the male pronucleus of mouse zygotes by direct microinjection. Some of these zygotes developed into mature mice which contained this gene and appeared to be producing a rabbit globin. Evidence for the presence of the gene in these animals was provided by Southern blot hybridization analysis. Evidence for the expression of the rabbit gene in these transformed mice and their offspring was provided by hemoglobin isoelectric focusing analysis and specific serological reactivity between mouse anti-rabbit hemoglobin antiserum and a hemolysate from the mice that developed from the microinjected zygotes. The use of this zygote transformation may allow the introduction and expression of a broad range of genetic elements in mammals.

我们通过直接显微注射将编码兔β-珠蛋白的基因转移到小鼠受精卵的雄原核中。其中一些受精卵发育成了含有该基因的成熟小鼠，并且似乎在产生兔珠蛋白。Southern印迹杂交分析为这些动物中该基因的存在提供了证据。血红蛋白等电聚焦分析以及小鼠抗兔血红蛋白抗血清与显微注射受精卵发育而来的小鼠的溶血产物之间的特异性血清学反应，为兔基因在这些转基因小鼠及其后代中的表达提供了证据。这种受精卵转化技术的应用可能会使多种遗传元件在哺乳动物中得以导入和表达。