100个高度信息丰富的人类简单序列重复多态性的分离与染色体定位

Isolation and chromosomal assignment of 100 highly informative human simple sequence repeat polymorphisms.

作者信息

Hudson T J, Engelstein M, Lee M K, Ho E C, Rubenfield M J, Adams C P, Housman D E, Dracopoli N C

机构信息

Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.

出版信息

Genomics. 1992 Jul;13(3):622-9. doi: 10.1016/0888-7543(92)90133-d.

DOI:10.1016/0888-7543(92)90133-d

PMID:1639389

Abstract

One hundred highly informative simple sequence repeat (SSR) polymorphisms have been isolated and mapped to specific human chromosomes by somatic cell hybrid analysis. These markers include 97 (CA)n, 2 (AGAT)n, and a single (AACT)n repeat. All the SSRs have heterozygosities greater than 0.50 and can be amplified using identical PCR conditions. At least one SSR was detected on every chromosome, except for chromosomes 22 and Y. The frequency of (CA)n repeats on each chromosome was proportional to the relative chromosomal length, except for chromosome 15, on which a substantial excess of markers was identified.

摘要

通过体细胞杂交分析，已分离出100个信息丰富的简单序列重复（SSR）多态性，并将其定位到特定的人类染色体上。这些标记包括97个（CA）n、2个（AGAT）n和1个（AACT）n重复序列。所有的SSR杂合度均大于0.50，并且可以在相同的PCR条件下进行扩增。除了22号和Y染色体外，在每条染色体上至少检测到一个SSR。除15号染色体外，每条染色体上（CA）n重复序列的频率与相对染色体长度成正比，在15号染色体上鉴定出大量过量的标记。