Norouzy A, Nassiry M R, Eftekhari Shahrody F, Javadmanesh A, Mohammad Abadi M R, Sulimova G E
Excellence Animal Science Center, College of Agriculture, Ferdowsi University of Mashhad, P.O. Box 91775-1163, Mashhad, Iran.
Genetika. 2005 Dec;41(12):1697-701.
BLAD is a hereditary disease in Holstein dairy cattle. The defective allele of CD18 gene which is responsible for this disease has a recessive inheritance. The recessive homozygous form (BL/BL) is lethal and since carrier animals have viability, BLAD frequency increases by use of carrier bulls in Artificial Insemination (AI). BLAD carriers can be detected easily by means of polymerase chain reaction followed by restriction analysis of the amplicons. In this study DNA samples from Holstein (n = 30) and Brown Swiss (n = 10) bulls from Abbas Abad AI center (Khorasan state of Iran) were analysed. A 101 bp fragment from the polymorphic region of CD18 gene located on chromosome 1 was amplified by PCR. Restriction enzymes TaqI and HaeIII were used to identify genotypes. Digestion products were screened by electrophoresis on 8% non-denaturing polyacrylamide gel and visualized by ethidium bromide staining. Frequencies of BL/TL (carrier) genotypes in Holstein and Brown Swiss bulls were 3.33% and 0%, respectively. Our pedigree studies of the carrier bull in this experiment revealed that the mutation inherited to him from Hawkeye bull (CANM 369995, BL). Although the elimination of BLAD-carrier bulls from the Holstein world would be the most efficient method to control this genetic disorder, many BLAD-carrier bulls are still listed commercially for AI and BLAD is still occurring in Iran. Monitoring the prevalence of BLAD-carriers in random selected herds may be helpful in judging the effectiveness of the BLAD-control program.
牛白细胞粘附缺陷症（BLAD）是荷斯坦奶牛的一种遗传性疾病。导致这种疾病的CD18基因缺陷等位基因具有隐性遗传特性。隐性纯合形式（BL/BL）是致死的，由于携带该基因的动物具有生存能力，在人工授精（AI）中使用携带该基因的公牛会使BLAD的频率增加。通过聚合酶链反应，随后对扩增产物进行限制性分析，可以很容易地检测出BLAD携带者。在本研究中，对来自伊朗霍拉桑省阿巴斯阿巴德人工授精中心的荷斯坦公牛（n = 30）和瑞士褐牛公牛（n = 10）的DNA样本进行了分析。通过PCR扩增位于1号染色体上CD18基因多态性区域的一个101 bp片段。使用限制性内切酶TaqI和HaeIII来鉴定基因型。消化产物在8%非变性聚丙烯酰胺凝胶上进行电泳筛选，并用溴化乙锭染色进行可视化观察。荷斯坦公牛和瑞士褐牛公牛中BL/TL（携带者）基因型的频率分别为3.33%和0%。我们对本实验中携带该基因的公牛的系谱研究表明，该突变是从鹰眼公牛（CANM 369995，BL）遗传给他的。尽管从荷斯坦牛群中淘汰携带BLAD基因的公牛是控制这种遗传疾病最有效的方法，但许多携带BLAD基因的公牛仍在商业上用于人工授精，并且BLAD在伊朗仍有发生。监测随机选择的牛群中BLAD携带者的流行情况可能有助于判断BLAD控制计划的有效性。
