大鼠肝脏中聚（ADP - 核糖基）组蛋白的自然存在。
Natural occurrence of poly(ADP-ribosyl) histones in rat liver.
作者信息
Ueda K, Omachi A, Kawaichi M, Hayaishi O
出版信息
Proc Natl Acad Sci U S A. 1975 Jan;72(1):205-9. doi: 10.1073/pnas.72.1.205.
Poly(ADP-ribose) bound to histones has been isolated from rat liver. When [14C]ribose was administered intraperitoneally to rats at a dosage of 300-750 mug (100-250 muCi)/10o g, approximately 1% of the radioactivity was recovered in the acid (5% CLCCCOOH)-INSOLUBLE MATERIAL OF THE LIVER NUCLEI 2 HR AFTER INJECTION. Of the acid-insoluble radioactivity, 4.5-9% was extractable with 0.25 N HCL. Carboxymethyl-cellulose column chromatography of the HCl-extracted material revealed that the radioactivity cochromatographed with histone subfractions f1 and, to a lesser extent, f2 and f3. Part of the protein-bound radioactivity was rendered acid-soluble by treatment with either snake venom phosphodiesterase or neutral NH2OH. From the enzyme digest, 5'-AMP and psiADP-ribose [2'-(5"-phosphoribosyl)-5'-AMP] were recovered, while the NH2OH treatment yielded ADP-ribose monomer and, presumably, oligomer. These observations indicate that ADP-ribose is attached to histones in vivo and is present both as a monomer and a polymer.
已从大鼠肝脏中分离出与组蛋白结合的聚（ADP - 核糖）。当以300 - 750微克（100 - 250微居里）/100克的剂量向大鼠腹腔注射[14C]核糖时，注射后2小时，肝脏细胞核的酸（5% 三氯乙酸）不溶性物质中回收了约1%的放射性。在酸不溶性放射性物质中，4.5 - 9%可用0.25N盐酸提取。对盐酸提取物进行羧甲基纤维素柱色谱分析表明，放射性与组蛋白亚组分f1共色谱，在较小程度上与f2和f3共色谱。部分与蛋白质结合的放射性通过用蛇毒磷酸二酯酶或中性NH2OH处理而变为酸溶性。从酶消化物中回收了5'-AMP和ψADP - 核糖[2'-(5"-磷酸核糖基)-5'-AMP]，而NH2OH处理产生了ADP - 核糖单体以及推测的寡聚物。这些观察结果表明，ADP - 核糖在体内与组蛋白相连，并且以单体和聚合物形式存在。
Zotero 插件