Mukundan Srinivasan, Ghaghada Ketan B, Badea Cristian T, Kao Chen-Yu, Hedlund Laurence W, Provenzale James M, Johnson G Allan, Chen Emmanuel, Bellamkonda Ravi V, Annapragada Ananth
Department of Radiology, Duke University Medical Center, Box 3808, Durham, NC 27705, USA.
AJR Am J Roentgenol. 2006 Feb;186(2):300-7. doi: 10.2214/AJR.05.0523.
The goal of this study was to determine if an iodinated, liposomal contrast agent could be used for high-resolution, micro-CT of low-contrast, small-size vessels in a murine model.
A second-generation, liposomal blood pool contrast agent encapsulating a high concentration of iodine (83-105 mg I/mL) was evaluated. A total of five mice weighing between 20 and 28 g were infused with equivalent volume doses (500 microL of contrast agent/25 g of mouse weight) and imaged with our micro-CT system for intervals of up to 240 min postinfusion. The animals were anesthetized, mechanically ventilated, and vital signs monitored allowing for simultaneous cardiac and respiratory gating of image acquisition.
Initial enhancement of about 900 H in the aorta was obtained, which decreased to a plateau level of approximately 800 H after 2 hr. Excellent contrast discrimination was shown between the myocardium and cardiac blood pool (650-700 H). No significant nephrogram was identified, indicating the absence of renal clearance of the agent.
The liposomal-based iodinated contrast agent shows long residence time in the blood pool, very high attenuation within submillimeter vessels, and no significant renal clearance rendering it an effective contrast agent for murine vascular imaging using a micro-CT scanner.
本研究的目的是确定一种碘化脂质体造影剂是否可用于小鼠模型中低对比度、小尺寸血管的高分辨率微型计算机断层扫描（micro-CT）。
评估了一种第二代脂质体血池造影剂，其包封了高浓度碘（83 - 105 mg I/mL）。总共五只体重在20至28克之间的小鼠被注入等量体积剂量（500微升造影剂/25克小鼠体重），并在注入后长达240分钟的时间间隔内用我们的微型计算机断层扫描系统进行成像。动物被麻醉、机械通气并监测生命体征，以便在图像采集时同时进行心脏和呼吸门控。
主动脉初始增强约900 H，2小时后降至约800 H的平台水平。心肌和心脏血池之间显示出良好的对比度差异（650 - 700 H）。未发现明显的肾影，表明该造影剂无肾清除现象。
基于脂质体的碘化造影剂在血池中显示出较长的停留时间，在亚毫米血管内具有非常高的衰减，且无明显肾清除，使其成为使用微型计算机断层扫描扫描仪进行小鼠血管成像的有效造影剂。
