超灵敏1型人类免疫缺陷病毒p24抗原检测法经改良后可用于干血斑检测，作为一种可靠且经济实惠的婴儿诊断检测方法。
Ultrasensitive human immunodeficiency virus type 1 p24 antigen assay modified for use on dried whole-blood spots as a reliable, affordable test for infant diagnosis.
作者信息
Patton Janet C, Sherman Gayle G, Coovadia Ashraf H, Stevens Wendy S, Meyers Tammy M
机构信息
Wits Paediatric HIV Unit, Wits Health Consortium, University of the Witwatersrand, 7 York Rd., Parktown 2193, Johannesburg, South Africa.
出版信息
Clin Vaccine Immunol. 2006 Jan;13(1):152-5. doi: 10.1128/CVI.13.1.152-155.2006.
Abstract
The ultrasensitive human immunodeficiency virus (HIV) p24 antigen assay was modified for use on pediatric dried whole-blood spots on Whatman no. 1 filter paper. The modified assay was found to be reliable and accurate, making it an affordable tool for pediatric HIV diagnosis in developing countries.
摘要
超敏人类免疫缺陷病毒（HIV）p24抗原检测方法经过改良，可用于检测Whatman 1号滤纸采集的儿科干血斑样本。改良后的检测方法被证明可靠且准确，使其成为发展中国家儿科HIV诊断的一种经济实惠的工具。
相似文献
1
Ultrasensitive human immunodeficiency virus type 1 p24 antigen assay modified for use on dried whole-blood spots as a reliable, affordable test for infant diagnosis.超灵敏1型人类免疫缺陷病毒p24抗原检测法经改良后可用于干血斑检测，作为一种可靠且经济实惠的婴儿诊断检测方法。
Clin Vaccine Immunol. 2006 Jan;13(1):152-5. doi: 10.1128/CVI.13.1.152-155.2006.
2
3
Ultrasensitive quantitative HIV-1 p24 antigen assay adapted to dried plasma spots to improve treatment monitoring in low-resource settings.适用于干血浆斑的超灵敏定量HIV-1 p24抗原检测法，以改善资源匮乏地区的治疗监测。
J Clin Virol. 2006 May;36(1):64-7. doi: 10.1016/j.jcv.2005.12.005. Epub 2006 Jan 23.
4
Ultrasensitive p24 antigen assay for diagnosis of perinatal human immunodeficiency virus type 1 infection.用于诊断围产期1型人类免疫缺陷病毒感染的超敏p24抗原检测法。
J Clin Microbiol. 2007 Jul;45(7):2274-7. doi: 10.1128/JCM.00813-07. Epub 2007 May 2.
5
Diagnosis of human immunodeficiency virus type 1 infection in infants by use of dried blood spots and an ultrasensitive p24 antigen assay.利用干血斑和超灵敏p24抗原检测法诊断婴儿1型人类免疫缺陷病毒感染
J Clin Microbiol. 2009 Feb;47(2):459-62. doi: 10.1128/JCM.01181-08. Epub 2008 Dec 10.
6
Prospective evaluation of amplification-boosted ELISA for heat-denatured p24 antigen for diagnosis and monitoring of pediatric human immunodeficiency virus type 1 infection.用于诊断和监测儿童1型人类免疫缺陷病毒感染的热变性p24抗原扩增增强酶联免疫吸附测定的前瞻性评估。
J Infect Dis. 1999 Oct;180(4):1089-95. doi: 10.1086/315012.
7
p24 antigen detection on dried blood spots is a feasible and reliable test for infant HIV infection in rural Tanzania.在坦桑尼亚农村地区，干血斑上的p24抗原检测是一种用于婴儿HIV感染的可行且可靠的检测方法。
Int J STD AIDS. 2011 Dec;22(12):719-21. doi: 10.1258/ijsa.2009.009382.
8
Evaluation of the ultrasensitive human immunodeficiency virus type 1 (HIV-1) p24 antigen assay performed on dried blood spots for diagnosis of HIV-1 infection in infants.对用于诊断婴儿1型人类免疫缺陷病毒（HIV-1）感染的干血斑超敏HIV-1 p24抗原检测法的评估。
Clin Vaccine Immunol. 2008 Feb;15(2):388-91. doi: 10.1128/CVI.00265-07. Epub 2007 Dec 5.
9
Sensitivity and specificity of complexed p24 antigen assay for early diagnosis of perinatal HIV-1 infection.
AIDS. 1993 Oct;7(10):1391-3.
10
Evaluation of the Murex HIV Ag/Ab Combination assay when used with dried blood spots.使用干血斑时对Murex HIV抗原/抗体联合检测法的评估。
Clin Microbiol Infect. 2007 Nov;13(11):1134-6. doi: 10.1111/j.1469-0691.2007.01809.x. Epub 2007 Aug 24.
引用本文的文献
1
Cross-Subtype Detection of HIV-1 Capsid p24 Antigen Using a Sensitive Europium Nanoparticle Assay.使用灵敏的铕纳米颗粒检测法对HIV-1衣壳p24抗原进行交叉亚型检测。
AIDS Res Hum Retroviruses. 2019 Apr;35(4):396-401. doi: 10.1089/AID.2018.0163. Epub 2019 Jan 14.
2
The Diagnosis of HIV Infection in Infants and Children.婴幼儿和儿童HIV感染的诊断
Iran J Pathol. 2016 Spring;11(2):89-96.
3
Standardization of a cytometric p24-capture bead-assay for the detection of main HIV-1 subtypes.用于检测主要HIV-1亚型的细胞计数p24捕获珠分析法的标准化
J Virol Methods. 2016 Apr;230:45-52. doi: 10.1016/j.jviromet.2016.01.009. Epub 2016 Jan 22.
4
Effectiveness of Early Infant Diagnosis (EID) in Detecting the Serostatus of HIV-Exposed Infants and Children.早期婴儿诊断（EID）在检测暴露于HIV的婴儿和儿童血清状态方面的有效性。
J Obstet Gynaecol India. 2015 Oct;65(5):315-9. doi: 10.1007/s13224-014-0632-2. Epub 2014 Oct 29.
5
Comparison of the Gen-Probe Aptima HIV-1 and Abbott HIV-1 qualitative assays with the Roche Amplicor HIV-1 DNA assay for early infant diagnosis using dried blood spots.使用干血斑对Gen-Probe Aptima HIV-1和雅培HIV-1定性检测法与罗氏Amplicor HIV-1 DNA检测法进行早期婴儿诊断的比较。
J Clin Virol. 2014 Aug;60(4):418-21. doi: 10.1016/j.jcv.2014.05.012. Epub 2014 Jun 2.
6
An overview of the clinical use of filter paper in the diagnosis of tropical diseases.滤纸在热带病诊断中的临床应用概述。
Am J Trop Med Hyg. 2014 Feb;90(2):195-210. doi: 10.4269/ajtmh.13-0463. Epub 2013 Dec 23.
7
Analysis of the optimal cut-point for HIV-p24 antigen testing to diagnose HIV infection in HIV-exposed children from resource-constrained settings.分析资源有限环境下 HIV 暴露儿童中 HIV-p24 抗原检测诊断 HIV 感染的最佳截断值。
J Clin Virol. 2011 Apr;50(4):338-41. doi: 10.1016/j.jcv.2011.01.012. Epub 2011 Feb 16.
8
Using a simplified human immunodeficiency virus type 1 p24 antigen assay to diagnose pediatric HIV-infection in Malawi.采用简化的人类免疫缺陷病毒 1 型 p24 抗原检测方法诊断马拉维儿童 HIV 感染。
J Clin Virol. 2010 Dec;49(4):299-302. doi: 10.1016/j.jcv.2010.08.013. Epub 2010 Sep 19.
9
Nucleic acid assay system for tier II laboratories and moderately complex clinics to detect HIV in low-resource settings.用于二级实验室和中等复杂诊所的核酸检测系统，用于在资源匮乏环境中检测 HIV。
J Infect Dis. 2010 Apr 15;201 Suppl 1(Suppl 1):S46-51. doi: 10.1086/650388.
10
Dried-plasma transport using a novel matrix and collection system for human immunodeficiency virus and hepatitis C virus virologic testing.使用新型基质和采集系统进行干燥血浆运输用于人类免疫缺陷病毒和丙型肝炎病毒的病毒学检测。
J Clin Microbiol. 2009 May;47(5):1491-6. doi: 10.1128/JCM.02354-08. Epub 2009 Mar 25.
本文引用的文献
1
Detection and quantification of human immunodeficiency virus type 1 p24 antigen in dried whole blood and plasma on filter paper stored under various conditions.在不同条件下储存的滤纸上干燥全血和血浆中人类免疫缺陷病毒1型p24抗原的检测与定量
J Clin Microbiol. 2005 Aug;43(8):3901-5. doi: 10.1128/JCM.43.8.3901-3905.2005.
2
Pediatric human immunodeficiency virus screening in an African district hospital.非洲地区医院的儿科人类免疫缺陷病毒筛查
Clin Diagn Lab Immunol. 2005 Jan;12(1):86-92. doi: 10.1128/CDLI.12.1.86-92.2005.
3
Evaluation of an ultrasensitive p24 antigen assay as a potential alternative to human immunodeficiency virus type 1 RNA viral load assay in resource-limited settings.在资源有限的环境中，评估超灵敏p24抗原检测作为1型人类免疫缺陷病毒RNA病毒载量检测潜在替代方法的可行性。
J Clin Microbiol. 2005 Jan;43(1):506-8. doi: 10.1128/JCM.43.1.506-508.2005.
4
PMTCT from research to reality--results from a routine service.预防母婴传播：从研究到实际应用——一项常规服务的结果
S Afr Med J. 2004 Apr;94(4):289-92.
5
Affordable diagnosis of human immunodeficiency virus infection in infants by p24 antigen detection.通过p24抗原检测对婴儿进行经济实惠的人类免疫缺陷病毒感染诊断。
Pediatr Infect Dis J. 2004 Feb;23(2):173-6. doi: 10.1097/01.inf.0000109332.83246.1a.
6
HIV-1 p24 antigen is a significant inverse correlate of CD4 T-cell change in patients with suppressed viremia under long-term antiretroviral therapy.在长期抗逆转录病毒治疗下病毒血症得到抑制的患者中，HIV-1 p24抗原与CD4 T细胞变化呈显著负相关。
J Acquir Immune Defic Syndr. 2003 Jul 1;33(3):292-9. doi: 10.1097/00126334-200307010-00002.
7
Whole blood collected on filter paper provides a minimally invasive method for assessing human transferrin receptor level.采集在滤纸上的全血为评估人类转铁蛋白受体水平提供了一种微创方法。
J Nutr. 2002 Dec;132(12):3760-3. doi: 10.1093/jn/132.12.3760.
8
Use of filter paper for the collection and analysis of human whole blood specimens.使用滤纸采集和分析人类全血标本。
J Nutr. 2001 May;131(5):1631S-6S. doi: 10.1093/jn/131.5.1631S.
9
Use of HIV-1 p24 as a sensitive, precise and inexpensive marker for infection, disease progression and treatment failure.
Int J Antimicrob Agents. 2000 Dec;16(4):441-5. doi: 10.1016/s0924-8579(00)00272-7.
10
Performance of five different assays for the quantification of viral load in persons infected with various subtypes of HIV-1. Swiss HIV Cohort Study.五种不同检测方法对感染不同亚型HIV-1者病毒载量的定量检测。瑞士HIV队列研究。
J Acquir Immune Defic Syndr. 2000 Feb 1;23(2):138-44. doi: 10.1097/00126334-200002010-00005.
Zotero 插件