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超灵敏1型人类免疫缺陷病毒p24抗原检测法经改良后可用于干血斑检测，作为一种可靠且经济实惠的婴儿诊断检测方法。

Ultrasensitive human immunodeficiency virus type 1 p24 antigen assay modified for use on dried whole-blood spots as a reliable, affordable test for infant diagnosis.

作者信息

Patton Janet C, Sherman Gayle G, Coovadia Ashraf H, Stevens Wendy S, Meyers Tammy M

机构信息

Wits Paediatric HIV Unit, Wits Health Consortium, University of the Witwatersrand, 7 York Rd., Parktown 2193, Johannesburg, South Africa.

出版信息

Clin Vaccine Immunol. 2006 Jan;13(1):152-5. doi: 10.1128/CVI.13.1.152-155.2006.

DOI:10.1128/CVI.13.1.152-155.2006
PMID:16426014
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1356617/
Abstract

The ultrasensitive human immunodeficiency virus (HIV) p24 antigen assay was modified for use on pediatric dried whole-blood spots on Whatman no. 1 filter paper. The modified assay was found to be reliable and accurate, making it an affordable tool for pediatric HIV diagnosis in developing countries.

摘要

超敏人类免疫缺陷病毒（HIV）p24抗原检测方法经过改良，可用于检测Whatman 1号滤纸采集的儿科干血斑样本。改良后的检测方法被证明可靠且准确，使其成为发展中国家儿科HIV诊断的一种经济实惠的工具。

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Ultrasensitive human immunodeficiency virus type 1 p24 antigen assay modified for use on dried whole-blood spots as a reliable, affordable test for infant diagnosis.超灵敏1型人类免疫缺陷病毒p24抗原检测法经改良后可用于干血斑检测，作为一种可靠且经济实惠的婴儿诊断检测方法。
Clin Vaccine Immunol. 2006 Jan;13(1):152-5. doi: 10.1128/CVI.13.1.152-155.2006.
2
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本文引用的文献

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Detection and quantification of human immunodeficiency virus type 1 p24 antigen in dried whole blood and plasma on filter paper stored under various conditions.在不同条件下储存的滤纸上干燥全血和血浆中人类免疫缺陷病毒1型p24抗原的检测与定量
J Clin Microbiol. 2005 Aug;43(8):3901-5. doi: 10.1128/JCM.43.8.3901-3905.2005.
2
Pediatric human immunodeficiency virus screening in an African district hospital.非洲地区医院的儿科人类免疫缺陷病毒筛查
Clin Diagn Lab Immunol. 2005 Jan;12(1):86-92. doi: 10.1128/CDLI.12.1.86-92.2005.
3
Evaluation of an ultrasensitive p24 antigen assay as a potential alternative to human immunodeficiency virus type 1 RNA viral load assay in resource-limited settings.在资源有限的环境中，评估超灵敏p24抗原检测作为1型人类免疫缺陷病毒RNA病毒载量检测潜在替代方法的可行性。
J Clin Microbiol. 2005 Jan;43(1):506-8. doi: 10.1128/JCM.43.1.506-508.2005.
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PMTCT from research to reality--results from a routine service.预防母婴传播：从研究到实际应用——一项常规服务的结果
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Affordable diagnosis of human immunodeficiency virus infection in infants by p24 antigen detection.通过p24抗原检测对婴儿进行经济实惠的人类免疫缺陷病毒感染诊断。
Pediatr Infect Dis J. 2004 Feb;23(2):173-6. doi: 10.1097/01.inf.0000109332.83246.1a.
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HIV-1 p24 antigen is a significant inverse correlate of CD4 T-cell change in patients with suppressed viremia under long-term antiretroviral therapy.在长期抗逆转录病毒治疗下病毒血症得到抑制的患者中，HIV-1 p24抗原与CD4 T细胞变化呈显著负相关。
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Whole blood collected on filter paper provides a minimally invasive method for assessing human transferrin receptor level.采集在滤纸上的全血为评估人类转铁蛋白受体水平提供了一种微创方法。
J Nutr. 2002 Dec;132(12):3760-3. doi: 10.1093/jn/132.12.3760.
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Use of filter paper for the collection and analysis of human whole blood specimens.使用滤纸采集和分析人类全血标本。
J Nutr. 2001 May;131(5):1631S-6S. doi: 10.1093/jn/131.5.1631S.
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Use of HIV-1 p24 as a sensitive, precise and inexpensive marker for infection, disease progression and treatment failure.
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Performance of five different assays for the quantification of viral load in persons infected with various subtypes of HIV-1. Swiss HIV Cohort Study.五种不同检测方法对感染不同亚型HIV-1者病毒载量的定量检测。瑞士HIV队列研究。
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