Chen I L, Yates R D
Cell Tissue Res. 1975;157(2):227-38. doi: 10.1007/BF00222068.
The Golgi complex in the Sertoli cell of the Syrian hamster is well developed and consists of stacks of cisternae and associated vesicles. The inner- and outermost cisternae of the Golgi stacks are usually moderately dilated and exhibit numerous fenestrations. The middle portions of the intermediate cisternae are greatly flattened and not fenestrated, but toward the periphery these cisternae gradually become dilated and show a few fenestrations. On the inner aspect of the Golgi stacks the following structures are seen frequently: (1) one or two series of linearly arrayed circular profiles some of which are interconnected by tubules; (2) networks of anastomosing tubules with circular or oval meshes (800 to 1200 A in diameter); and/or (3) irregularly disposed tubules. The circular profiles and tubules are approximately 450 A in diameter. Acid phosphatase activity was localized in these anastomosing tubules when the tissues were incubated for more than one hour in a modified Gomori's medium (Barka and Anderson, 1963). Strong thiamine pyrophosphatase activity was demonstrated in the inner one to three cisternae of the Golgi stacks but not in the associated tubules. The system of the Golgi associated tubules is morphologically and histochemically distinct from the Golgi stacks and is probably equivalent to the Golgi-endoplasmic reticulum-lysosome system (GERL) in other cell types. The three dimensional aspects of the GERL-equivalent system are discussed.
叙利亚仓鼠支持细胞中的高尔基体发育良好,由一系列扁平囊泡和相关的小泡组成。高尔基体堆叠的最内层和最外层扁平囊泡通常适度扩张,并呈现出许多窗孔。中间扁平囊泡的中部极度扁平且无窗孔,但向周边这些扁平囊泡逐渐扩张并出现一些窗孔。在高尔基体堆叠的内侧经常可以看到以下结构:(1)一到两排呈线性排列的圆形轮廓,其中一些通过小管相互连接;(2)具有圆形或椭圆形网孔(直径800至1200埃)的吻合小管网络;和/或(3)不规则排列的小管。圆形轮廓和小管的直径约为450埃。当组织在改良的Gomori培养基(Barka和Anderson,1963)中孵育超过一小时时,酸性磷酸酶活性定位于这些吻合小管中。在高尔基体堆叠的内侧一到三个扁平囊泡中显示出强烈的硫胺素焦磷酸酶活性,但在相关的小管中未显示。高尔基体相关小管系统在形态学和组织化学上与高尔基体堆叠不同,可能相当于其他细胞类型中的高尔基体-内质网-溶酶体系统(GERL)。本文讨论了GERL等效系统的三维结构。