Toniolo D, Basilico C
Cell. 1975 Mar;4(3):255-62. doi: 10.1016/0092-8674(75)90173-7.
We have isolated temperature-sensitive SV40-transformed 3T3 cells which are unable to grow in low or depleted serum at the nonpermissive temperature. At 39 degrees C, these cells do not grow in 1 percent serum, but they grow if the serum concentration is raised to 10 percent. At 32 degrees they grow in both serum concentrations. This phenotype seems to be due to a cellular mutation, as the virus rescued from these cells is wild-type. We tested whether other characteristics of transformed cells were expressed in a temperature sensitive way. While high saturation density is ts in these cells, other parameters of transformation are expressed at both temperatures. In addition, when these cells are incubated in low serum at 39 degrees C, they keep synthesizing DNA and lose viability very fast, while under the same conditions normal 3T3 cells remain viable for long times and are unable to initiate DNA synthesis. These cells therefore do not appear to revert to a normal phenotype at the high temperature, and they are more likely to represent transformed cell variants with a temperature-dependent serum requirement.
我们分离出了温度敏感的SV40转化的3T3细胞，这些细胞在非允许温度下于低血清或血清耗尽的环境中无法生长。在39摄氏度时，这些细胞在1%血清中不生长，但如果血清浓度提高到10%，它们就能生长。在32摄氏度时，它们在两种血清浓度下都能生长。这种表型似乎是由于细胞突变所致，因为从这些细胞中拯救出的病毒是野生型的。我们测试了转化细胞的其他特征是否以温度敏感的方式表达。虽然这些细胞的高饱和密度是温度敏感的，但转化的其他参数在两个温度下都有表达。此外，当这些细胞在39摄氏度下于低血清中孵育时，它们会持续合成DNA并很快失去活力，而在相同条件下，正常的3T3细胞能长时间保持活力且无法启动DNA合成。因此，这些细胞在高温下似乎不会恢复到正常表型，它们更有可能代表具有温度依赖性血清需求的转化细胞变体。
